Ten male football referees will be recruited to perform two intermittent football protocols , separated by at least 7 days (wash-out period). After passing the eligibility criteria, the participants will be randomly assigned, according to a computer-generated allocation schedule, to 1 of 2 beverages sequences: (1) intervention - menthol solution 0.01% (room temperature) and (2) placebo - noncaloric berry-flavoured solution (room temperature). The participants will be provided with one of the 2 beverages before warm-up (pre-cooling) and at the half-time (per-cooling). The trials will follow a randomised counterbalanced crossover design, blinded to the participants, and will take place in indoor facilities, where WBGT exceed 30◦C, at the same time of the day, to control for circadian variations. Each trial involves an exercise protocol (SAFT-90), lasting 90 minutes, separated into two 45-minute parts. The first half will be preceded by a warm-up and the second half by a 15-minute break. The results of this study are expected to determine whether mouth rinsing a menthol solution, before a football exercise protocol performed in the heat, will help to alleviate physiological strain and improve performance parameters, comparing to a non-cooling strategy, in elite male football referees. Thus, we can be closer to defining nutritional strategies of internal cooling, that will be an advantage for the performance of the football referees, concretely in the competitions carried out under adverse environmental conditions.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
DOUBLE
Enrollment
10
Menthol application, through oral solutions, stimulates the mandibular and maxillary branches of the trigeminal nerve (which are predominantly responsible for detection of temperature and nociceptive stimuli across the face and within the oral cavity) and has consistently shown to improve thermal comfort and decrease thermal sensation, which are thought to modulate perceived exertion to improve performance in the heat.
Heart rate change
Heart rate is a physiological parameter of performance and will be measured through HR monitors (Polar H10 Heart Rate Sensor, USA). PolarH10 can accurately measure mean HR and low-frequency oscillations (up to 0.15 Hz) of HR at rest and during exercise.
Time frame: A change in heart rate from baseline (immediately before exercise) to each 15-minute block of the exercise protocol will be assessed.
Sweating rate at the first half
Sweating rate is a physiological parameter of performance and is going to be calculated recurring to the following equation : SR= (Pre exercise body weight - postexercise body weight + fluid intake - urine volume)/(exercise time in hours).
Time frame: Sweating rate is going to be calculated at the end of the first half of the exercise protocol (after 45 min of exercise)
Sweating rate at the second half
Sweating rate is a physiological parameter of performance and is going to be calculated recurring to the following equation : SR= (Pre exercise body weight - postexercise body weight + fluid intake - urine volume)/(exercise time in hours).
Time frame: Sweating rate is going to be calculated at the end of the second half of the exercise protocol (after 90 min of exercise)
Core temperature change
Core temperature is a physiological parameter of performance and can be accessed using a telemetric pill ingested 30 minutes prior the start of the exercise (BodyCap®, France). Due to the good measuring accuracy, the ability to measure in field-based situations and the non-invasive character of this temperature measurement method, the ingestible telemetric temperature pill is suitable to assess core temperature during exercise
Time frame: A change in core temperature from baseline (immediately before exercise) to each 15-minute block of the exercise protocol will be assessed
Ratings of perceived exertion (RPE)
RPE is a subjective psychological/perceptual parameter that impacts performance and will be recorded through CR-10 Borg scale that goes from 0 ("rest") to 10 ("maximal effort").
Time frame: RPE is going to be accessed at the end of the exercise protocol.
Thermal comfort (TC) change
TC is a subjective psychological/perceptual parameter that influences performance and is going to be accessed according to a 6-point scale from to -3 ("very uncomfortable) to 3 ("very comfortable").
Time frame: A change in TC from baseline (immediately before intervention) to after intervention and to each 15-minute block of the exercise protocol will be assessed.
Thermal sensation (TS) change
TS is a subjective psychological/perceptual parameter that impacts performance and will be recorded with a 9-point scale from -4 ("very cold") to 4 ("very warm").
Time frame: A change in TS from baseline (immediately before intervention) to after intervention and to each 15-minute block of the exercise protocol will be assessed.
Perceived thirst (PT) change
PT is a subjective marker of hydration status and will be evaluated recurring to a 7-point scale from 1 ("not thirsty at all") to 7 ("very, very thirsty")
Time frame: A change in PT from baseline (immediately before intervention) to after intervention and to each 15-minute block of the exercise protocol will be assessed.
Physical performance change
Physical performance responses will be measured using 100 Hz accelerometery, with Sonda 4.0 software (StatSports®, UK) to estimate total distance, sprint distance, accelerations and decelerations number
Time frame: A change in each physical performance indicator from baseline (immediately after the warm-up) to each 15-minute block of the exercise protocol will be assessed
Hydration status before
Hydration status is going to be assessed recurring to urine test strip (Combur10 Test M, Roche) and Urisys 1100® analyser (Roche, Switzerland).
Time frame: Hydration status is going to be recorder at baseline (pre-exercise)
Hydration status after
Hydration status is going to be assessed recurring to urine test strip (Combur10 Test M, Roche) and Urisys 1100® analyser (Roche, Switzerland).
Time frame: Hydration status is going to be recorder at the end of the exercise protocol
Ad libitum fluid intake at the first half
Fluid intake is going to be recorded via the measurement of mass change of the individual bottles provided to the referees at the warm-up and at the end of the session, to the nearest 0.1 ml (Seca, Hamburg, Germany).
Time frame: Ad libitum fluid intake is going to be assessed at the end of the first half of the exercise protocol (after 45 min of exercise)
Ad libitum fluid intake at the second half
Fluid intake is going to be recorded via the measurement of mass change of the individual bottles provided to the referees at the warm-up and at the end of the session, to the nearest 0.1 ml (Seca, Hamburg, Germany).
Time frame: Ad libitum fluid intake is going to be assessed at the end of the second half of the exercise protocol (after 90 min of exercise)
Blood lactate levels before
Blood lactate levels will be measured recurring to a Blood Lactate Meter (Lactate Pro 2, Arkray, U.S.A).
Time frame: Blood lactate levels are going to be recorded before the start of the exercise protocol
Blood glucose levels before
Blood glucose levels will be measured recurring to a Glucometer (FreeStyle Precision Neo, Abbott Laboratories, U.S.A).
Time frame: Blood glucose levels are going to be recorded before the start of the exercise protocol
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Blood glucose levels after
Blood glucose levels will be measured recurring to a Glucometer (FreeStyle Precision Neo, Abbott Laboratories, U.S.A).
Time frame: Blood glucose levels are going to be recorded immediately after the end of the exercise protocol
Blood lactate levels after
Blood lactate levels will be measured recurring to a Blood Lactate Meter (Lactate Pro 2, Arkray, U.S.A).
Time frame: Blood lactate levels are going to be recorded immediately after the end of the exercise protocol