Endocan Biomarker Level in Gingival Crevicular Fluid in Periodontitis Patients With Type 2 Diabetes Mellitus

Alexandria University42 enrolled

Overview

Aim of the current study was to evaluate Endocan biomarker level in gingival crevicular fluid of patients with stage 1 or 2 (mild to moderate) periodontitis with controlled type 2 diabetes mellitus (DM) and compare it to that in DM free periodontitis patients.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Periodontitis Diabetes Mellitus

Eligibility

Sex: ALLMin age: 40 YearsMax age: 65 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Probing pocket depths (PPD) ≤ 5 mm. * Clinical attachment level (CAL) ≤ 4 mm. * Type 2 DM, for at least the past 3 years. * HbA1c ≤ 7%. Exclusion Criteria: * Uncontrolled Diabetes Mellitus. * Systemic antibiotic or anti-inflammatory medication use in the previous 2 months. * Non-surgical periodontal therapy in the previous 6 months. * Surgical periodontal therapy in the previous 12 months. * Use of calcium channel blockers, phenytoin, or cyclosporine. * Pregnancy. * Smokers

Locations (1)

Faculty of Dentistry, Alexandria University

Alexandria, Egypt

Outcomes

Primary Outcomes

Endocan level

Endocan level in gingival crevicular samples was assessed by commercially available ELISA kit (Bioneovan Co., Ltd, Elisa Human Endocan/esm-1 ELISA Kit. Beijing, China). Assays were performed according to the manufacturer's instructions. Color change will be measured with a microplate reader (Hellma GmbH \& Co. KG. Müllheim, Germany) at 450 nm. Concentrations were determined based on the respective assay standard curve. All samples were run in duplicate and values will be averaged

Time frame: Day 1

Pocket probing depth (PPD)

PPD was assessed at six different sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, and disto-lingual) per tooth at all teeth present, except third molars. PPD was assessed using Williams calibrated periodontal probe and recordings were made to the nearest mm; observations close to 0.5 mm were rounded to the upper whole mm.

Time frame: Day 1

Clinical Attachment Level (CAL)

CAL was assessed at six different sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, and disto-lingual) per tooth at all teeth present, except third molars. CAL was assessed using Williams calibrated periodontal probe and recordings were made to the nearest mm; observations close to 0.5 mm were rounded to the upper whole mm.

Time frame: Day 1

Percentage of bleeding on probing sites (BOP%)

BOP% was assessed at six different sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, and disto-lingual) per tooth at all teeth present, except third molars. BOP% was assessed using Williams calibrated periodontal probe and the number of sites with BOP were divided by the total number of examined sites and multiplied by 100 to calculate the percentage

Time frame: Day 1

Data from ClinicalTrials.gov

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