We intend to establish an efficient method for plasma cfDNA extraction and Bisulfite transformation to facilitate the detection of DNA methylation status using multiplex fluorescence PCR. Meanwhile, we expect to identify several plasma methylation markers that can be highly sensitive for multi-cancer detection. Finally, we will provide a pan-cancer blood test that is easy to operate, low cost, accurate and easy to promote.
Study Type
OBSERVATIONAL
Enrollment
3,000
Cell-free DNA were extracted from plasma collected from individuals of cancer and non-cancerous disease groups, then the DNA was bisulfite converted and tested by DNA methylation assay based on real-time PCR platform.
The first affiliated hospital of Zhengzhou University
Zhengzhou, Henan, China
RECRUITINGSensitivity
The reference standard is the results of histopathological tests
Time frame: immediately after the procedure
Specificity
Time frame: immediately after the procedure
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