Selecting Specific Bio-markers and Researching Mechanisms of Immune Regulation From Inhalation Injury and Traumatic Lung Injury Based on Single Cell Sequencing Technology

Inclusion Criteria: 1. Lung injury caused by burn trauma is confirmed, and the patient is hospitalized within 24 hours after burn or trauma (the clinician can make a definite diagnosis of lung injury based on the comprehensive judgment of the patient's etiology, history, clinical manifestations, blood gas analysis, laboratory tests and imaging); 2. Patients with inhalation injury caused by head, face and neck burns or inhalation injury combined with multiple body burns \< 30%; 3. Patients with simple inhalation injury; 4. The normal control group should have no smoking history and be normal population; 5. According to the provisions of Good Clinical Practice(GCP), the informed consent has been obtained and the informed consent has been signed to volunteer for the test. Exclusion Criteria: 1. Basic pulmonary diseases like chronic obstructive pulmonary disease (COPD), asthma and et al; 2. Severe organ dysfunction; 3. Autoimmune diseases; 4. Tumor; 5. Severe infection; 6. Pregnant or nursing women; 7. Fatal or life-threatening events caused by trauma; 8. Any history which may interfere the results or increase the risk of patients judged by researchers.