The investigators will determine the acute effect of exercise on breastmilk composition. Participants will come in to the lab on three different days, out of which they will exercise on two of these days and rest in the lab on the third day. The conditions will be randomly allocated to each participant. Breastmilk samples will be obtained in the morning of each test day, as well as immediately after, one hour after and four hours after the exercise/rest condition. Dietary intake will be standardized on the test days.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
32
Treadmill walking/running with moderate intensity
Treadmill walking/running with high intensity
Department of circulation and medical imaging , NTNU
Trondheim, Norway
Breastmilk metabolite composition
Global metabolomics profiling using MS/MS technology
Time frame: Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours)
Complex lipids composition
Global metabolomics profiling using MS/MS technology
Time frame: Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours)
Adiponectin
Concentration
Time frame: Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours)
Leptin
Concentration
Time frame: Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours)
Insulin Growth Factor-1
Concentration
Time frame: Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours)
Insulin
Concentration
Time frame: Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours)
Ghrelin
Concentration
Time frame: Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours)
Cytokine profiling (Multiplex 27)
Concentration
Time frame: Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours)
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