In this project the investigators will test if it is possible to measure changes in intestinal gas production after supplementation of a complex fiber mixture over a 36 hour period in both lean normoglycemic individuals and individuals with insulin resistance and/or prediabetes with overweight when compared with a placebo Changes in intestinal gas production will also be related to energy expenditure, substrate metabolism, microbial composition and related metabolites in feces, blood and urine.
In this project the investigators aim to test whether it is possible to precisely measure the changes in fermentation gas patterns after supplementation of a complex fibre mixture over a 36-hour period in both lean normoglycemic and individuals with insulin resistance and/or prediabetes with overweight when compared with a placebo. Additionally, the investigators will investigate whether changes in fermentation patterns will be directly related to changes in carbohydrate and fat oxidation and to microbial composition or related metabolites and hormones in blood, faeces, and urine.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
28
Participants will undergo a two day supplementation of a complex fiber supplement vs. placebo with a two-week washout period between the two interventions in a randomized order
Participants will undergo a two day supplementation of a complex fiber supplement vs. placebo with a two-week washout period between the two interventions in a randomized order
Department of Human Biology, Maastricht University Medical Centre
Maastricht, Netherlands
RECRUITINGMaastricht University Medical Centre
Maastricht, Netherlands
RECRUITING24 hour intestinal gas concentrations
changes in intestinal gas excretion (methane, hydrogen or hydrogen sulphide will be measured using gas sensors in a modified respiration chamber)
Time frame: after ingestion of the supplement up until 24 hours after supplementation continuously
24 hour energy expenditure
changes in 24 hour energy expenditure will be measured in a respiration chamber
Time frame: after ingestion of the supplement up until 24 hours after supplementation continuously
24 hour substrate metabolism
changes in 24 hour substrate metabolism will be measured in a respiration chamber (fat and carbohydrate oxidation)
Time frame: after ingestion of the supplement up until 24 hours after supplementation continuously
fecal microbial metabolite concentrations
the concentrations of fecal SCFA and BCFAs will be measured
Time frame: after ingestion of the supplement and 24 hours after supplementation
Fecal microbiota composition
Faecal microbiota composition will be assessed via16S rRNA gene sequencing
Time frame: at baseline and 24 hours after supplementation
24 hour glucose monitoring
24 hour glucoses concentrations will be measured using a wearable monitor during each testday
Time frame: after ingestion of the supplement up until 24 hours after supplementation
circulating blood markers
plasma blood glucose, insulin, GLP-1, glucagon, PYY, triglycerides, FFA, SCFA and BCFA
Time frame: after ingestion of the supplement at multiple timepoints up until 24 hours after supplementation
Urine metabolites
urinary concentrations of phenolic compounds, p-cresol and nitrogen will be measured
Time frame: at baseline up until 24 hours after supplementation
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