Modulation of Ciliogenesis in Glioma Stem Cells

Fondazione Policlinico Universitario Agostino Gemelli IRCCS100 enrolled

Overview

The study aims at investigating the cilium-related transcriptome in patients-derived glioblastoma stem cells and the potential impact of modulation of cilium players in vitro, in vivo and ex vivo in glioblastoma brain organoids. Moreover, drugs inhibiting cilia disassembly will be tested. Finally, the potential prognostic role of a cilium-related gene expression signature in glioblastoma will be assessed.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

100

Conditions

Glioblastoma Glioma, Malignant

Interventions

Biological sample collectionOTHER

Collection of tumor tissue and blood for stem cell culture and organoids generation.

Dissecting ciliogenesis playersOTHER

Assessment of cilium-related transcriptome in glioblastoma stem cells. Modulation of cilium-related genes and administration of cilium-targeted drugs to glioblastoma stem cells in vitro and ex vivo in glioblastoma brain organoids.

Validating ciliogenesis playersDIAGNOSTIC_TEST

Cilium-related signature will be studied in tumor tissue to evaluate its prognostic role.

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: To be enrolled in the study patients must: 1. Have a radiological diagnosis of supratentorial glioblastoma, or 2. Have a radiological diagnosis of first recurrence of a primary supratentorial glioblastoma (for which a formal histopathologic diagnosis of glioblastoma had been made at first surgery), according with RANO criteria (Wen, 2010); 3. Be a candidate to neurosurgery for glioblastoma at the Operational Unit of Neurosurgery FPG; 4. Be of an age of 18 years or above; 5. Provide written informed consent for participation to the study. Exclusion criteria To be enrolled in the study patients must not: 1. Have not enough pathological material removed at surgery available both for mandatory routine histopathological diagnosis and for the present study, as judged by the Principal Investigator; 2. Have not a definitive pathological diagnosis of a primary supratentorial GBM, according with 2016 WHO classification.

Locations (1)

Fondazione Policlinico Universitario A. Gemelli IRCCS

Rome, Italy

Outcomes

Primary Outcomes

Biological

Correlation between modulation of cilia disassembly complex genes, and tumor growth and invasion in brain organoids.

Time frame: Through study completion, an average of 2 years

Data from ClinicalTrials.gov

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