Multiomic Analysis of Traumatic Brain Injury and Hypertension Intracranial Hemorrhage Lesion Tissue

RenJi Hospital10 enrolled

Overview

The goal of this experimental observation study is to figure out differently expressed biomarkers in lesion tissues in traumatic brain injury or hypertension intracranial hemorrhage patients. The main questions it aims to answer is: * Which RNA, protein and metabolites are differently expressed in lesion tissues? * What molecular mechanism is participated in TBI or ICH? Participants will be treated by emergency operation, and their lesion tissues will be collected during the operation.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Brain Injury Traumatic Severe Intracranial Hemorrhage, Hypertensive

Interventions

Type of diseaseOTHER

Different types of pathogen: brain injury by trauma or intracranial hemorrhage due to hypertension.

Eligibility

Sex: ALLMin age: 16 YearsMax age: 65 Years

Medical Language ↔ Plain English

Inclusion Criteria: 1. Age \>18 and \<65 2. Within 12 hours after brain injury 3. GCS ≥3 and ≤8 4. Closed brain injury with frontotemporal contusion or basal ganglia intracranial hemorrhage 5. Need emergency intracranial hematoma evacuating operation 6. Patient's agents are informed and consent the research Exclusion Criteria: 1. With TBI, stroke, ICH or intracranial tumor history 2. Death within 24 hours 3. Immunosuppression state 4. Severe organ dysfunction 5. Complicated infective disease 6. Pregnant

Locations (1)

Brain Injury Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University

Shanghai, Shanghai Municipality, China

RECRUITING

Outcomes

Primary Outcomes

RNA biomarkers in TBI or ICH patients' lesion tissues assessed by transcriptome bioinformatic analysis

Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed by bioinformatic analysis to reveal different RNA expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers.

Time frame: Immediately after collecting tissues

Protein biomarkers in TBI or ICH patients' lesion tissues assessed by proteomic bioinformatic analysis

Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed by bioinformatic analysis to reveal different protein expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers.

Time frame: Immediately after collecting tissues

Metabolite biomarkers in TBI or ICH patients' lesion tissues assessed by metabolomic bioinformatic analysis

Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed by bioinformatic analysis to reveal different metabolite expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers.

Time frame: Immediately after collecting tissues

Central Contacts

Zhenghui He, MD

CONTACT

+8613585916217 hezhenghui8@hotmail.com

Data from ClinicalTrials.gov

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