The goal of this experimental observation study is to figure out differently expressed biomarkers in body fluid in traumatic brain injury patients. The main questions it aims to answer is: * Which RNA, protein and metabolites are differently expressed in lesion tissues? * What molecular mechanism is participated in TBI? Participants will be treated by emergency operation, and their body fluid samples will be collected in emergency room and during the operation.
Study Type
OBSERVATIONAL
Enrollment
10
Different types of pathogen: brain injury by trauma or hydrocephalus due to various diseases.
Brain Injury Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Shanghai, Shanghai Municipality, China
RECRUITINGRNA biomarkers in patients' blood & cerebrospinal fluid assessed by transcriptome bioinformatic analysis
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed and compared with control group to filter potential RNA biomarkers in blood and cerebrospinal fluid of severe TBI patients.
Time frame: Immediately after collecting blood & cerebrospinal fluid
Protein biomarkers in patients' blood & cerebrospinal fluid assessed by proteomic bioinformatic analysis
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed and compared with control group to filter potential protein biomarkers in blood and cerebrospinal fluid of severe TBI patients.
Time frame: Immediately after collecting blood & cerebrospinal fluid
Metabolite biomarkers in patients' blood & cerebrospinal fluid assessed by metabolomic bioinformatic analysis
Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed and compared with control group to filter potential metabolite biomarkers in blood and cerebrospinal fluid of severe TBI patients.
Time frame: Immediately after collecting blood & cerebrospinal fluid
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