The goal of this observational study is to evaluate and to correlate melatonin levels in plasma, saliva and cerebrospinal fluid (CSF) samples of patients undergoing lumbar puncture diagnostic for specific neurological diseases.
Study Type
OBSERVATIONAL
Enrollment
36
Saliva was collected using Salivettes (Sarsted, Newton, NC, USA) in dim light. CSF was obtained in the morning after overnight fasting by lumbar puncture at the L3/L4 or L4/L5 interspace. Blood samples were drawn in fasting conditions. Plasma was separated by centrifugation.
Istituto Auxologico Italiano - S. Giuseppe Hospital
Verbania, VB, Italy
RECRUITINGMelatonin levels in saliva
Analysis of melatonin levels by ELISA kit. Values expressed in pg/ml
Time frame: Baseline
Melatonin levels in plasma
Analysis of melatonin levels by ELISA kit. Values expressed in pg/ml
Time frame: Baseline
Melatonin levels in cerebrospinal fluid
Analysis of melatonin levels by ELISA kit. Values expressed in pg/ml
Time frame: Baseline
Orexin levels in cerebrospinal fluid
Analysis of orexin levels by ELISA kit. Values expressed in ng/ml
Time frame: Baseline
Neurofilaments light chain levels (NFL) in cerebrospinal fluid
Analysis of NFL levels by SIMOA kit. Values expressed in pg/ml
Time frame: Baseline
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