Study on Endobronchial Ultrasound-guided Transbronchial Mediastinal Cryobiopsy Via a Tunnel in the Diagnosis of Mediastinal Lymphadenopathy

Inclusion Criteria: * Patients with at least one mediastinal and/or hilar lymphadenopathy (≥1cm in the short axis) in the absence of a known or suspected primary lung cancer, requiring diagnostic bronchoscopy. * The selected patients should complete the routine examination of EBUS-TBNA/EBUS-TBMC via a tunnel before operation, such as blood routine examination, coagulation function, electrocardiogram, chest CT, etc. * There was no contraindication of puncture and cryobiopsy. * Fully informed of the purpose and method of the study, agreed to participate in the study, and signed the informed consent form. Exclusion Criteria: * The lesion is a mediastinal cyst or abscess. * The patient is allergic to lidocaine and midazolam. * The site to be biopsied has a high risk of bleeding detected by Doppler and/or contrast CT such as bronchial artery penetration or suspected lung metastasis of renal cancer. * Unstable angina pectoris, congestive heart failure, severe bronchial asthma * The patient did not agree to participate in this study * Participation in other studies within three months without withdrawal or termination will affect the observation of this study * The researcher believes that there is any person who is not suitable for the selection