Study of Long Non-coding RNA SNHG15 as a Novel Biomarker in HBV Associated HCC

N/ANot Yet RecruitingNCT05840133

Qianfoshan Hospital156 enrolled

Overview

In this study, the investigators will detect the expression of HBV-related HCC biomarker lncRNA SNHG15 in tumor tissues and peripheral blood, to explore the specific molecular markers for the early diagnosis of HBV-related HCC.

Paired hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) and adjacent liver tissues were selected, and lncRNA SNHG15 was detected by RT-PCR. At the same time, healthy subjects were selected as the control group, and lncRNA SNHG15 in blood was detected by RT-PCR. At the same time, the correlation between lncRNA SNHG15 and the clinical data of patients, such as diagnosis, pathological grading, recurrence, metastasis and survival time was statistically analyzed.

Study Type

OBSERVATIONAL

Enrollment

156

Conditions

HCC

Interventions

No intervention was required for patients or control group in this studyDIAGNOSTIC_TEST

The patient was treated normally and no intervention was required in this study

Eligibility

Sex: ALLMin age: 18 YearsMax age: 80 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * The age and sex of the healthy control group were matched with that of HBV-associated patient group. There was no tumor in the other parts of the body, and no tumor in the blood system. The healthy control group did not have any liver benign diseases. There are no inflammatory diseases in other parts of the body; the functions of the liver, kidney, and heart were normal. Exclusion Criteria: * The volunteer has tumors in the liver or other parts of the body, or blood system tumors; The volunteer has benign liver diseases; The patient has inflammatory disease elsewhere. If the volunteer has any one of the above diseases, it shall be excluded.

Outcomes

Primary Outcomes

Detection of lncRNA SNHG15 in HBV-associated HCC patients before surgery

The expression of lncRNA SNHG15 in blood samples: The expressions of lncRNA SNHG15 in blood samples were detected by real-time fluorescence quantitative PCR method.

Time frame: 1 to 3 days before surgery

Secondary Outcomes

Detection of lncRNA SNHG15 in HBV-associated HCC patients 1 week after surgery

1. The expressions of lncRNA SNHG15 in tissues: In the tumor and adjacent tissues from HBV-associated HCC patients, the expressions of lncRNA SNHG15were measured by real-time fluorescence quantitative PCR method. 2. The expression of lncRNA SNHG15 in blood samples: The expressions of lncRNA SNHG15 in blood samples were detected by real-time fluorescence quantitative PCR method.

Time frame: one week after surgery

Detection of lncRNA SNHG15 in HBV-associated HCC patients at 6 months after surgery

The expression of lncRNA SNHG15 in blood samples: The expressions of lncRNA SNHG15 in blood samples were detected by real-time fluorescence quantitative PCR method.

Time frame: 6 months postoperatively

Detection of lncRNA SNHG15 in the control groups as baseline

The expression of lncRNA SNHG15 in blood samples: The expressions of lncRNA SNHG15 in blood samples were detected by real-time fluorescence quantitative PCR method.

Time frame: the level of lncRNA SNHG15 were detected as baseline

Central Contacts

Chunqing Wang, Dr.

CONTACT

053189269680 1150156766@qq.com

Data from ClinicalTrials.gov

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