This research project, approved and funded, is part of a larger project, entitled "Healthy Soil for Food", which completes several work packages, one of which is a clinical research submitted to Ethics Committee entitled "O-LIVE: Impact of olive oil Portuguese in biomarkers of healthy volunteers". The main objective of this work is to evaluate the impact of ingestion of an olive oil rich in polyphenols on clinical biomarkers and parameters, such as anthropometric measurements, on inflammatory gene expression regulation and on the composition of the intestinal microbiota.
Olive oil is an ancient product that is crucial for social cohesion and for local economies in the Mediterranean region, particularly in Portugal. There is ample evidence on the protective effect of olive oil in cardiovascular disease, diabetes, oncological diseases and neurological degeneration. Furthermore, olive oil has been shown to increase the rate of oxidation, inhibiting lipogenesis and stimulating lipolysis, in addition to affecting the expression of receptors involved in weight regulation and fat use. These health benefits of olive oil derive from its specific nutritional composition, with monounsaturated fatty acids, polyphenols and phenolic compounds, which act on metabolism, modulation of inflammatory responses and gene expression. But the composition of olive oil can vary considerably depending on the type of cultivation of olive trees, soil management practices, as well as the extraction and technological processes involved in olive oil production. In this work the investigators will use an olive oil rich in polyphenols (produced in a sustainable and local manner) and evaluate its effect on several important clinical parameters involved in chronic diseases. The investigators anticipate that the results of this work will help support the relationship between consuming higher polyphenol olive oil and improved overall health.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
OTHER
Masking
NONE
Enrollment
Participants will add to their usual diet a daily intake of 30 mL of an olive oil rich in polyphenols for 3-months (100 days)
Escola Superior de Biotecnologia, Universidade Católica Portuguesa
Porto, Portugal
RECRUITINGBody weight
Body weight will be assessed using a scale
Time frame: Day 1
Body weight
Body weight will be assessed using a scale
Time frame: Day 50-60
Body weight
Body weight will be assessed using a scale
Time frame: Day 100
Bacterial populations in fecal cultures
Bacterial populations in fecal cultures will be analysed by microbial DNA extraction and quantitative real-time polymerase-chain reaction (PCR) using specific primers.
Time frame: Day 1
Bacterial populations in fecal cultures
Bacterial populations in fecal cultures will be analyzed by microbial DNA extraction and quantitative real-time polymerase-chain reaction (PCR) using specific primers.
Time frame: Day 100
Inflammatory gene expression
Saliva sample will also be collected for subsequent DNA extraction and application of quantitative real-time PCR to determine the expression of inflammatory genes.
Time frame: Day 1
Inflammatory gene expression
Saliva sample will also be collected for subsequent DNA extraction and application of quantitative real-time PCR to determine the expression of inflammatory genes.
Time frame: Day 100
Body composition (% of fat mass and % of non-fat mass)
Body composition will be assessed using Body Impedance Analysis (body composition analyser)
Time frame: Day 1
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Body composition (% of fat mass and % of non-fat mass)
Body composition will be assessed using Body Impedance Analysis (body composition analyser)
Time frame: Day 100
Adhesion to mediterranean diet
PREDIMED (PREvencion con Diet MEDiterranea) is a brief 14-item tool able to capture a strong monotonic inverse association between adherence to a good quality dietary pattern (Mediterranean diet) and obesity indexes in a population of adults (doi:10.1371/journal.pone.0043134)
Time frame: Day 1
Adhesion to mediterranean diet
PREDIMED (PREvencion con Diet MEDiterranea) is a brief 14-item tool able to capture a strong monotonic inverse association between adherence to a good quality dietary pattern (Mediterranean diet) and obesity indexes in a population of adults (doi:10.1371/journal.pone.0043134)
Time frame: Day 50-60
Adhesion to mediterranean diet
PREDIMED (PREvencion con DIeta MEDiterranea) is a brief 14-item tool able to capture a strong monotonic inverse association between adherence to a good quality dietary pattern (Mediterranean diet) and obesity indexes in a population of adults (doi:10.1371/journal.pone.0043134)
Time frame: Day 100
Lipid profile serum level
A drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for total cholesterol, HDL, LDL and triacylglycerols
Time frame: Day 1
Lipid profile serum level
A drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for total cholesterol, HDL, LDL and triacylglycerols
Time frame: Day 100
C-reactive Protein serum level
A drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for PCR levels
Time frame: Day 1
C-reactive Protein serum level
A drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for PCR levels
Time frame: Day 100
glycated hemoglobin A1c serum levels
drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for levels glycated hemoglobin (ha1c)
Time frame: Day 1
glycated hemoglobin A1c serum levels
drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for levels glycated hemoglobin (ha1c)
Time frame: Day 100