The investigators present a weight loss diet intervention study, to be conducted as a within-subject design, with all food and beverages provided, to assess interaction of non-nutritive sweetener (sucralose) with a high-fibre weight loss diet, on markers of gut health in humans. This study will allow assessment of the effects of a non-nutritive sweetener (sucralose) with a high-fibre (soluble fibre, fructo-oligosaccharides, FOS) diet on metabolic health and activity and composition of gut microbiota, by a controlled human diet intervention study. The investigators propose to recruit participants living with obesity, with a poor diet quality (moderate habitual fibre intake) to additionally address diet inequalities in the research approach, and this will also allow examine the time-course of adaptation of the gut microbiome (measured in faecal samples). The investigators will also assess changes in free-living glycaemic control with addition of dietary fibre and bio-markers of health.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
20
Control diet (30% Fat, 15% Protein, 55% Carbohydrate, Fibre rising from 10 to 16g/d). Fed to energy balance (1.5 x Resting Metabolic Rate, RMR).
High Fibre Weight Loss diet (30% Fat, 15% Protein, 55% Carbohydrate). Fed to energy deficit (100% RMR). The quantity of fructo-oligosaccharides (FOS) will be fixed at 20g/day for all kcal levels with additional fibre coming from the diet provided. The FOS will be split over breakfast, lunch and dinner in drinks/yoghurts which will be compulsory to consume.
High Fibre and Non-Nutritive Sweetener Weight Loss diet (30% Fat, 15% Protein, 55% Carbohydrate). Fed to energy deficit (100% RMR). The quantity of fructo-oligosaccharides (FOS) and non-nutritive sweetener (sucralose) will be fixed at 20g/day and 30mg/day respectively for all kcal levels with additional fibre coming from the diet provided. The FOS and sucralose will be split over breakfast, lunch and dinner in drinks/yoghurts which will be compulsory to consume.
Rowett Institute, University of Aberdeen
Aberdeen, United Kingdom
Change in gut metabolites (from faecal samples) in response to dietary fibre and non-nutritive sweetener
Measured by changes in faecal short chain fatty acids concentrations
Time frame: At baseline and end of each arm (study days 1, 15, 29 and 43)
Change in gut microbiome composition (from faecal samples) in response to dietary fibre and non-nutritive sweetener
Measured by changes in bacterial 16S ribosomal ribonucleic acid gene sequencing; 16S rRNA
Time frame: At baseline and end of each arm (study days 1, 15, 29 and 43)
Change in gut metabolite production (from faecal samples) in response to dietary fibre and non-nutritive sweetener
Measured by changes in calprotectin concentrations
Time frame: At baseline and end of each arm (study days 1, 15, 29 and 43)
Change in free-living glycaemic control in response to dietary fibre and non-nutritive sweetener
measured using continuous glucose monitoring (CGM)
Time frame: every day of study diets (42days)
Change in glycaemic control in response to dietary fibre and non-nutritive sweetener
measured by changes in fasted plasma glucose and insulin
Time frame: At baseline and end of each arm (study days 1, 15, 29 and 43)
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