Production of Gut Microbiota-Derived Metabolites in Response to Different Fatty Acid Profiles

Laval University49 enrolled

Overview

The proposed project mainly aims to investigate the microbial processes leading to dietary metabolites production, independently of long-term microbiota adaptation to the diet, by measuring the microbiota-derived metabolite production from a meal sequence rich in saturated fatty acids (SFA) or the same meals but rich in polyunsaturated fatty acids (PUFA) in individuals with or without obesity and Type 2 diabetes (T2D)

The gut microbiota interacts with the host's enteric and systemic functions through the production of gut microbiota-derived metabolites. A subset of these metabolites, the endocannabinoids (eCB) and their congeners are derived from fatty acids and play a crucial role in metabolic health. The research project aims to study gut microbiota-derived metabolites, including the eCB and their congeners, in response to a 2-day diet rich in polyunsaturated fatty acids or rich in saturated fatty acids in individuals with or without abdominal obesity and type 2 diabetes. The investigators hope to better understand the kinetic production of circulating endocannabinoidome mediators derived from fatty acids in the diet.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Obesity, Abdominal Diabete Type 2

Interventions

Nutritionnal interventionOTHER

Participants will be provided with meals corresponding to each diet during the week. Participants will be required to eat only the meals provided.

Eligibility

Sex: ALLMin age: 45 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Men and women aged between 45 and 65 years, menopaused women ; * Body mass index between 20 to 35 kg/m² ; * General good health ; * Score of diet quality (HEI) between 40 and 70 ; * Computer and Internet access ; * Basic understanding of written French ; * Daily stool frequency. Exclusion Criteria: * Intestinal pathologies including inflammatory bowel disease and gastrointestinal cancers ; * Alcohol consumption greater than 15 portions for men and 10 for women weekly. * Active tobacco and cannabis usage ; * Consumption of omega-3 dietary supplements ; * Consumption of dietary supplements (vitamins and probiotics) - must be ceased 2 weeks before the dietary intervention ; * Completion of a course of antibiotics in the past 3 months ; * Important weight change (+/- 5 kg) in the past 6 months ; * Type 1 diabetes or insulin-treated diabetes.

Locations (1)

Institut sur la nutrition et les aliments fonctionnels - INAF

Québec, Quebec, Canada

Outcomes

Primary Outcomes

Fasting and postprandial lipid metabolite production in response to dietary lipid intervention in abdominal obesity and type 2 diabetes

Interindividual variations in the endocannabinoidome mediators levels following a 2-day SFA and UFA diet intervention according to abdominal adiposity and type 2 diabetes.

Time frame: 7 days

Secondary Outcomes

Gut microbiota in response to a 2 day dietary lipid intervention in abdominal obesity and type 2 diabetes

Interindividual variations in the gut microbiota composition following a 2-day SFA and UFA diet intervention according to abdominal adiposity and type 2 diabetes.

Time frame: 7 days

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.