The Efficacy and Safty of FMT in Patients With CID

Pittsburgh Sleep Quality Index，PSQI

The PSQI was used to assess the sleep quality of the participants in the last 1 month. It consists of 19 self-assessments and 5 self-assessment items, of which the 19th self-assessment item and 5 self-assessment items do not participate in the scoring. The 18 items formed 7 components, and each component was scored according to 0-3 levels. The cumulative score of PSQI total score ranged from 0 to 2 l. The higher the score, the worse the sleep quality

Time frame: baseline and 1-, 2-, 3-, 6- months post FMT

Insomnia Severity Index，ISI

ISI is a commonly used insomnia severity scale used to assess the extent and impact of individual insomnia in the past two weeks. The ISI consists of seven items, and individuals need to choose the answer best suitable for their condition on each item, and then aggregate the scores of all items to get the total score. The total score ranged from 0 to 28, with higher scores indicating higher insomnia severity. Each item has four answer options, used to describe the frequency of insomnia, severity and effects on the individual.

Time frame: baseline and 1-, 2-, 3-, 6- months post FMT

Bray-Curtis dissimilarity

Bray-Curtis dissimilarity is a beta diversity metric used to quantify differences in microbial community composition between two time points. A higher dissimilarity score indicates greater divergence in taxonomic profiles. This outcome measures the change in gut microbiota structure from baseline to 1-month after fecal microbiota transplantation (FMT), serving as an index of engraftment and microbial shift

Time frame: baseline and 1-month post FMT

Sleep onset latency (SL)

Based on polysomnography (mins)

Time frame: baseline and 1 month post FMT

Self-Rating Anxiety Scale (SAS)

Participants need to according to the past a period of time (usually in the past week or two weeks), choose a most accord with their feelings answer options, usually expressed by the following level: no or little time (1), part of the time (2 points), quite a large amount of time (3 points), most or all of the time (4 points). An initial assessment of individual anxiety was possible by calculating the total score.

Time frame: baseline and 1-, 2-, 3-, 6- months post FMT

Self-Rating Depression Scale (SDS)

It usually contains 20 topics and is widely used in research and clinical practice. Participants need to according to the past a period of time (usually in the past week or two weeks), choose a most accord with their feelings answer options, usually expressed by the following level: no or little time (1), part of the time (2), quite a large amount of time (3), most or all of the time (4). The total raw score ranges from 20 to 80, and is typically converted to a standard score by multiplying by 1.25 (range: 25 to 100), with higher scores indicating greater severity of depression.

Time frame: baseline and 1-, 2-, 3-, 6-months post FMT

Gastrointestinal Symptom Rating Scale，GSRS

The GSRS includes 15 questions about gastrointestinal symptoms covering a range of symptoms including abdominal pain, flatulence, nausea, vomiting, loss of appetite, etc.The score rang from 16-112, the less the better.

Time frame: baseline and 1-, 2-, 3-, 6-months post FMT

Arousal index (ArI)

Based on polysomnography (events/h)

Time frame: baseline and 1-month post FMT

Non-rapid eye movement sleep duration(NREM)

Based on polysomnography (mins)

Time frame: baseline and 1-month post FMT

Rapid eye movement sleep duration(REM)

Based on polysomnography (mins)

Time frame: baseline and 1-month post FMT

Observed OTUs

Observed OTUs (Operational Taxonomic Units) represent the count of unique taxa identified in a sample, reflecting species richness. This outcome measures the number of observed OTUs in fecal samples at baseline and 1-month post-FMT, indicating changes in microbial richness following intervention.

Time frame: baseline and 1-month post FMT

Shannon Diversity Index

The Shannon index is a commonly used α-diversity metric that accounts for both richness and evenness in microbial communities. Higher values reflect greater diversity. This outcome evaluates within-sample microbial diversity before and after FMT.

Time frame: baseline and 1-month post FMT

Simpson Diversity Index

The Simpson index measures the probability that two randomly selected individuals in a sample belong to the same species. It is sensitive to dominant taxa. This outcome assesses within-sample microbial diversity changes induced by FMT.

Time frame: baseline and 1-month post FMT

Chao1 Richness Estimator

The Chao1 index estimates microbial species richness by accounting for the number of rare taxa (singletons and doubletons). It provides a more robust estimate of total richness compared to observed OTUs. This outcome evaluates the estimated richness change after FMT.

Time frame: baseline and 1-month post FMT