This study aims to investigate the relationship between diet and the microbiota-gut-brain axis.
Dietary fibre is well-known for its many health benefits, including the support of gastrointestinal, metabolic, and mental health. Although studies investigating whole dietary patterns in relation to cognition have demonstrated that diet quality and a healthy dietary pattern are associated with better cognitive performance, the role of dietary fibre in this regard is understudied. It is now understood that the gut microbiota (trillions of microbes inhabiting the gastrointestinal tract) communicates bidirectionally with the brain to influence mental health and cognition. Importantly, dietary fibre has been shown to positively affect the microbiota composition. The aim of this study is to understand the effects of dietary fibre on the microbiota-gut-brain axis. Using a cross-sectional design, habitual low fibre (\<=18 grams/day, n=200), moderate fibre (18.1-24.9 grams/day, n=75), and high fibre (=\>25 g/day, n=75) consumers will be compared at baseline on measures of cognition, responses to acute and chronic stress, and biological markers of the microbiota-gut-brain axis. The investigators hypothesize that participants with higher dietary fibre intake at baseline will perform better in the cognitive tasks compared to individuals with low fibre intake, and that this difference can, in part, be mediated by the gut microbiota.
Study Type
OBSERVATIONAL
Enrollment
350
Habitual dietary consumption from a variety of foods will be assessed using 7-day food logs entered by the participant and assessed for accuracy by a dietitian.
APC Microbiome Ireland
Cork, Ireland
RECRUITINGTrait stress/mood: self-report
Self-report questionnaires
Time frame: Compared at baseline
Trait stress/mood: hypothalamic-pituitary-adrenal axis activity
Cortisol from saliva samples
Time frame: Compared at baseline
Responses to acute stress: self-report
Self-report questionnaires
Time frame: Compared at baseline
Responses to acute stress: hypothalamic-pituitary-adrenal axis activity
Cortisol from saliva samples
Time frame: Compared at baseline
Responses to acute stress: sympathetic-adrenal-medullary pathway activity
Galvanic skin response taken from the skin on the hand
Time frame: Compared at baseline
Cognitive performance: working memory
Spatial Working Memory
Time frame: Compared at baseline
Cognitive performance: episodic memory
Modified Rey Auditory Verbal Learning Test (ModRey)
Time frame: Compared at baseline
Cognitive performance: decision making
Iowa Gambling Task
Time frame: Compared at baseline
Cognitive performance: emotional inhibition
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Emotional stroop
Time frame: Compared at baseline
Cognitive performance: sustained attention
Rapid Visual Information Processing
Time frame: Compared at baseline
Cognitive performance: visual pattern recognition memory
Pattern Recognition Memory
Time frame: Compared at baseline
Cognitive performance: cognitive flexibility
Intra-Extra Dimensional Set Shifting
Time frame: Compared at baseline
Cognitive performance: social cognition
Emotion Recognition Task
Time frame: Compared at baseline
Cognitive performance: affective perceptual bias
Emotional Bias Task
Time frame: Compared at baseline
Microbiota composition and function
Shotgun metagenomics of fecal samples
Time frame: Compared at baseline
Microbial and host metabolites
Untargeted metabolomics analysis in fecal and urine samples
Time frame: Compared at baseline
Inflammation
Inflammatory markers in lipopolysaccharide stimulated and unstimulated bloods
Time frame: Compared at baseline