The goal of this pilot intervention is to learn about how a well-formulated ketogenic diet (WFKD) impacts various health factors in generally healthy adults. The main questions it aims to answer are: * Establish whether an 8-week isocaloric, WFKD improves body composition and metabolic biomarkers in adults without chronic disease. * Examine changes in transcriptomic sequencing pathways pre- and post-WFKD intervention. * Explore gut microbial changes in adults without chronic disease that consume a WFKD. Participants will follow a well-formulated ketogenic diet for 8-weeks. Study procedures include: * Weekly body weight tracking * Daily urinary ketone assessment * Pre/post stool samples for gut microbiota analyses * Pre/post DXA scans * Diet quality tracking through 3-day food records
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
PREVENTION
Masking
NONE
Enrollment
20
1:1 ketogenic diet that is focused on intake of high-quality fats (nuts/seeds, fatty fish, avocado) and reduction in processed food.
University of Kansas Medical Center
Kansas City, Kansas, United States
Changes in Body Composition-- dual x-ray absorptiometry (DXA)
Measures of tissue distribution
Time frame: Baseline, 8 Week
Changes in Waist Circumference (WC)
Waist circumference (cm)
Time frame: Baseline, 4 Week, 8 Week
Changes in Body Mass Index (BMI)
Ratio of height and weight in kg/m\^2
Time frame: Baseline, 4 Week, 8 Week
Changes in Homeostatic model assessment of Insulin Resistance (HOMA-IR)
Ratio of fasting glucose and fasting insulin with a conversion factor, unitless
Time frame: Baseline, 4 Week, 8 Week
Changes in Advanced Lipid Panel
Time frame: Baseline, 8 Week
Changes in Inflammation
Changes in high sensitivity C-reactive protein (hs-CRP) (mg/L)
Time frame: Baseline, 8 Week
Changes in transcriptomic sequencing pathways
Measured via RNASequencing (RNA-Seq) technique: high-throughput (\~25,000 genes) RNAseq using SE100 Illumina (HiSeq) reads
Time frame: Baseline, 8 weeks
Gut microbial changes
Taxonomic classification of individual operational taxonomic units (OTU) with 97% similarity and produce an OTU table of all identified taxa across all samples
Time frame: Baseline, 8 weeks
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