Outcomes of the Embryos of Different Ovarian Follicle Sizes, and the miRNA Detection in Their Blastocyst Culture

Acibadem University55 enrolled

Overview

In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.

In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Embryo Quality

Interventions

Oocyte pick-upPROCEDURE

With the needle, transvaginally USG-guided oocyte pick-up procedure is going to be performed

Eligibility

Sex: FEMALEMin age: 20 YearsMax age: 38 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Female patients aged between 20-38 who are planned to have IVF treatment * On the day of trigger before the oocyte pick-up, patients having at least 5, and 14 at most follicles exceeding 11mm * Patient having BMI below 30 Exclusion Criteria: * PCO patients * Patients having endometriosis * Patients having male factor with severe oligoazoospermia

Locations (1)

Acibadem University Atakent Hospital

Istanbul, Turkey (Türkiye)

RECRUITING

Outcomes

Primary Outcomes

Oocyte grading

Morphologic Oocyte grading will be analyzed right after the procedure due to different follicle sizes

Time frame: after each hyper stimulation process of the patients, when the follicle sizes are large enough to pick up which is roughly 10-15 days after the hyper stimulation has started

Day-3 Embryo Grading

Morphologic Day-3 embryo grading will be analyzed after the procedure due to different follicle sizes

Time frame: Three days after the intracytoplasminc sperm injection(ICSI) procedure

Day-5 Embryo Grading

Day-5 embryo grading(Gardner classification) will be analyzed after the procedure due to different follicle sizes

Time frame: 5 days after the intracytoplasminc sperm injection(ICSI) procedure

beta hCG results of the transferred embryos

Clinical pregnancy outcomes

Time frame: ten days after the embryo transfer

Secondary Outcomes

micro RNA expression in culture medium

In the culture medium of blastocysts are going to be analyzed for microRNA levels that are expressed

Time frame: Culture mediums of day 5 embryos will be collected to be studied, after the last embryo is obtained and all will be studied at the end of the recruitment

Central Contacts

NAZLI ALBAYRAK, MD

CONTACT

00905054111626 albayraknalzi@gmail.com

TURGUT AYDIN, MD

CONTACT

00905323722713 turgut.aydin@acibadem.com

Data from ClinicalTrials.gov

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