Today, it is difficult to predict liver function after transplantation and therefore livers where poor function is assumed (marginal livers) become discarded. The study aim is to increase the number of available donor livers, especially for liver cancer patients, by pre-treating and testing marginal ones (extended criteria donor (ECD) livers) liver on a liver perfusion machine. A liver perfusion machine can simulate liver transplantation and enables functional/quality testing before transplantation. The machine will hopefully also make marginal livers more functional by reducing ischemia- \& reperfusion injury. A marginal donor liver is perfused ex situ with oxygenated blood from a blood donor on a machine. The liver can be tested here for function using internationally recognized criteria. At the same time, the investigators will carry out analyzes with microdialysis which can give a better picture of organ function and damage. Additionally, various samples of the liver and perfusate will be collected. Liver that achieves criteria for transplantation will be offered to the recipient.
The study is a single-center, prospective, open-label cohort study designed to determine the efficacy of liver perfusion of marginal ECD livers on a perfusion machine before liver transplantation. Donor livers will be preserved with standard cooling followed by three perfusion modes (Dual hypothermic oxygenated machine perfusion (DHOPE) / Controlled oxygenated rewarming (COR) / Normothermic machine perfusion (NMP)), a method used internationally to pre-treating marginal livers before liver transplantation and which able testing of function and quality before a transplantation is performed. Only livers that fulfill international criteria (Groningen criteria) will be approved for Ltx. In this study microdialysis will be used to monitoring the liver during machine perfusion and up to 14 days in the postoperative course to monitor the function of the liver. The hypothesis is that microdialysis will provide a better prediction of liver function than the existing Groeningen criteria. The investigators expect that most included patients, who receive ECD livers perfused on a machine, will receive a postoperative well-functioning liver graft which entails a significant survival benefit. The study is designed as a cohort study because included patients have a very poor prognosis (median overall survival of approx. 6 months) and others treatment options are lacking. The primary objective is to establish definitive, objective criteria for pre-transplant liver function by defining a threshold value for microdialysis parameters (especially lactate) between donor livers that will restore function and those that will not during ex vivo machine perfusion in ECD livers, which are superior to established criteria (Groeningen and Viability testing and transplantation of marginal livers (VITTAL) criteria)). The primary endpoint is microdialysis lactate level between donor livers after 4 hours of normothermic machine perfusion in ECD livers. The follow-up period is 12 months.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
PREVENTION
Masking
NONE
Enrollment
22
Patients who receive an ECD liver after an ex-vivo liver perfusion where the liver has been tested for function and approved according to international functional criteria (Groeningen criteria)
Lactate level in microdialysate from hilar plate after normothermic machine perfusion - 4 hours
Lactate level in microdialysate from hilar plate after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Lactate level in microdialysate from liver tissue after normothermic machine perfusion - 2.5 hours
Microdialysis lactate level in donor liver tissue after 2.5 hours of normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Lactate level in microdialysate from liver tissue after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Microdialysis lactate level in donor liver tissue after 2 hours of Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Pyruvate level in microdialysate from liver tissue after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Microdialysis pyruvate level in donor liver tissue after 2 hours of Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Glycerol level in microdialysate from liver tissue after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Microdialysis glycerol level in donor liver tissue after 2 hours of Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Glucose level in microdialysate from liver tissue after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Microdialysis glucose level in donor liver tissue after 2 hours of Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Lactate level in microdialysate from liver tissue after controlled oxygenated rewarming machine perfusion - 1 hour
Microdialysis lactate level in donor livers tissue after 1 hour of controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Glycerol level in microdialysate from liver tissue after controlled oxygenated rewarming machine perfusion - 1 hour
Microdialysis glycerol level in donor livers tissue after 1 hour of controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Glucose level in microdialysate from liver tissue after controlled oxygenated rewarming machine perfusion - 1 hour
Microdialysis glucose level in donor livers tissue after 1 hour of controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Pyruvate level in microdialysate from liver tissue after controlled oxygenated rewarming machine perfusion - 1 hour
Microdialysis pyruvate level in donor livers tissue after 1 hour of controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Glucose level in microdialysate from liver tissue after normothermic machine perfusion - 2.5 hours
Microdialysis glucose level in donor liver tissue after 2.5 hours of normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Glucose level in microdialysate from liver tissue after normothermic machine perfusion - 4 hours
Microdialysis glucose level in donor liver tissue after 4 hours of normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Pyruvate level in microdialysate from liver tissue after normothermic machine perfusion - 2.5 hours
Microdialysis pyruvate level in donor liver tissue after 2.5 hours of normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Pyruvate level in microdialysate from liver tissue after normothermic machine perfusion - 4 hours
Microdialysis pyruvate level in donor liver tissue after 4 hours of normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Glycerol level in microdialysate from liver tissue after normothermic machine perfusion - 2.5 hours
Microdialysis glycerol level in donor liver tissue after 2.5 hours of normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Glycerol level in microdialysate from liver tissue after normothermic machine perfusion - 4 hours
Microdialysis glycerol level in donor liver tissue after 4 hours of normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Lactate level in microdialysate from bile after normothermic machine perfusion - 4 hours
Microdialysis lactate level from bile after normothermic machine perfusion - 4 hours in extended criteria donors livers.
Time frame: 4 hours
Lactate level in microdialysate from bile after normothermic machine perfusion - 2.5 hours
Microdialysis lactate level from bile after normothermic machine perfusion - 2.5 hours in extended criteria donors livers.
Time frame: 2.5 hours
Glucose level in microdialysate from bile after normothermic machine perfusion - 4 hours
Microdialysis glucose levels from bile after normothermic machine perfusion - 4 hours in extended criteria donors livers.
Time frame: 4 hours
Glucose level in microdialysate from bile after normothermic machine perfusion - 2.5 hours
Microdialysis glucose levels from bile after normothermic machine perfusion - 2.5 hours in extended criteria donors livers.
Time frame: 2.5 hours
Pyruvate level in microdialysate from bile after normothermic machine perfusion - 4 hours
Microdialysis pyruvate levels from bile after normothermic machine perfusion - 4 hours in extended criteria donors livers.
Time frame: 4 hours
Pyruvate level in microdialysate from bile after normothermic machine perfusion - 2.5 hours
Microdialysis pyruvate levels from bile after normothermic machine perfusion - 2.5 hours in extended criteria donors livers.
Time frame: 2.5 hours
Glycerol level in microdialysate from bile after normothermic machine perfusion - 4 hours
Glycerol levels from bile after normothermic machine perfusion - 4 hours in extended criteria donors livers.
Time frame: 4 hours
Glycerol level in microdialysate from bile after normothermic machine perfusion - 2.5 hours
Glycerol levels from bile after normothermic machine perfusion - 2.5 hours in extended criteria donors livers.
Time frame: 2.5 hours
Glycerol level in microdialysate from bile after controlled oxygenated rewarming machine perfusion - 1 hour
Glycerol levels from bile after controlled oxygenated rewarming machine perfusion - 1 hour - in extended criteria donors livers.
Time frame: 1 hour
Glucose level in microdialysate from bile after controlled oxygenated rewarming machine perfusion - 1 hour
Glucose levels from bile after controlled oxygenated rewarming machine perfusion - 1 hour - in extended criteria donors livers.
Time frame: 1 hour
Lactate level in microdialysate from bile after controlled oxygenated rewarming machine perfusion - 1 hour
Lactate levels from bile after controlled oxygenated rewarming machine perfusion - 1 hour - in extended criteria donors livers.
Time frame: 1 hour
Pyruvate level in microdialysate from bile after controlled oxygenated rewarming machine perfusion - 1 hour
Pyruvate levels from bile after controlled oxygenated rewarming machine perfusion - 1 hour - in extended criteria donors livers.
Time frame: 1 hour
Pyruvate level in microdialysate from bile after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Pyruvate levels from bile after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours - in extended criteria donors livers.
Time frame: 2 hours
Glycerol level in microdialysate from bile after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Glycerol levels from bile after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours - in extended criteria donors livers.
Time frame: 2 hours
Lactate level in microdialysate from bile after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Lactate levels from bile after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours - in extended criteria donors livers.
Time frame: 2 hours
Glucose level in microdialysate from bile after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Glucose levels from bile after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours - in extended criteria donors livers.
Time frame: 2 hours
Bile gases pH - 4 hours after normothermic machine perfusion
Bile gas pH - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases pH - 2.5 hours after normothermic machine perfusion
Bile gas pH - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases Lactate - 4 hours after normothermic machine perfusion
Bile gas Lactate - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases Lactate - 2.5 hours after normothermic machine perfusion
Bile gas Lactate - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases Glucose - 4 hours after normothermic machine perfusion
Bile gas Glucose - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases Glucose - 2.5 hours after normothermic machine perfusion
Bile gas Glucose - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases Oxygen (O2) - 4 hours after normothermic machine perfusion
Bile gas O2 - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases O2 - 2.5 hours after normothermic machine perfusion
Bile gas O2 - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases carbon dioxide (CO2) - 4 hours after normothermic machine perfusion
Bile gas CO2 - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases CO2 - 2.5 hours after normothermic machine perfusion
Bile gas CO2 - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases bicarbonate (HCO3) - 4 hours after normothermic machine perfusion
Bile gas HCO3 - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases HCO3 - 2.5 hours after normothermic machine perfusion
Bile gas HCO3 - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases base excess (BE) - 4 hours after normothermic machine perfusion
Bile gas BE - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Ischemia/reperfusion injury - 4 hours after normothermic machine perfusion
Ischemia/reperfusion injury in liver biopsies, graded according to Ali JM et al, Liver transplant 2015; nil, mild, moderate, and severe. - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Ischemia/reperfusion damage - 4 hours after normothermic machine perfusion
Ischemia/reperfusion damage in liver biopsies, graded according to Suzuki score (Tian Y et al, Stem Cell Research \& Therapy, 2016); score 0, 1, 2, 3, (0=none, 4= severe) - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Ischemia/reperfusion injury - after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Ischemia/reperfusion injury in liver biopsies, graded according to Tian Y et al et al, Liver transplant 2015; nil, mild, moderate, and severe. - after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Ischemia/reperfusion damage - after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Ischemia/reperfusion damage in liver biopsies, graded according to Suzuki score (Tian Y et al, Stem Cell Research \& Therapy, 2016); score 0, 1, 2, 3, (0=none, 4= severe) - after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Ischemia/reperfusion injury - before machine perfusion
Ischemia/reperfusion injury in liver biopsies, graded according to Ali JM et al, Liver transplant 2015; nil, mild, moderate, and severe. - before machine perfusion in extended criteria donors livers
Time frame: 1 minute
Ischemia/reperfusion damage - before machine perfusion
Ischemia/reperfusion damage in liver biopsies, graded according to Suzuki score (Tian Y et al, Stem Cell Research \& Therapy, 2016); score 0, 1, 2, 3, (0=none, 4= severe) - before machine perfusion in extended criteria donors livers
Time frame: 1 minute
Ischemia/reperfusion injury - after controlled oxygenated rewarming machine perfusion - 1 hour
Ischemia/reperfusion injury in liver biopsies, graded according to Ali JM et al, Liver transplant 2015; nil, mild, moderate, and severe - after controlled oxygenated rewarming machine perfusion - 1 hour in extended criteria donors livers
Time frame: 1 hour
Ischemia/reperfusion damage - after controlled oxygenated rewarming machine perfusion - 1 hour
Ischemia/reperfusion damage in liver biopsies, graded according to Suzuki score (Tian Y et al, Stem Cell Research \& Therapy, 2016); score 0, 1, 2, 3, (0=none, 4= severe) - after controlled oxygenated rewarming machine perfusion - 1 hour in extended criteria donors livers
Time frame: 1 hour
Ischemia/reperfusion injury - 2.5 hours after normothermic machine perfusion
Ischemia/reperfusion injury in liver biopsies, graded according to Ali JM et al, Liver transplant 2015; nil, mild, moderate, and severe. - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Ischemia/reperfusion damage - 2.5 hours after normothermic machine perfusion
Ischemia/reperfusion damage in liver biopsies, graded according to Suzuki score (Tian Y et al, Stem Cell Research \& Therapy, 2016); score 0, 1, 2, 3, (0=none, 4= severe) - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases BE - 2.5 hours after normothermic machine perfusion
Bile gas BE - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases oxygen saturation (SO2) - 4 hours after normothermic machine perfusion
Bile gas SO2- 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases SO2 - 2.5 hours after normothermic machine perfusion
Bile gas SO2- 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases hemoglobin (Hb)- 4 hours after normothermic machine perfusion
Bile gas Hb - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases Hb - 2.5 hours after normothermic machine perfusion
Bile gas Hb - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases sodium (Na) - 4 hours after normothermic machine perfusion
Bile gas Na - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases Na - 2.5 hours after normothermic machine perfusion
Bile gas Na - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases potassium (K) - 4 hours after normothermic machine perfusion
Bile gas K - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases K - 2.5 hours after normothermic machine perfusion
Bile gas K - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gases Calcium (Ca) - 4 hours after normothermic machine perfusion
Bile gas Ca - 4 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Bile gases Ca - 2.5 hours after normothermic machine perfusion
Bile gas Ca - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Markers of injury, inflammation - 2.5 hours after normothermic machine perfusion
Markers of injury, inflammation - 2.5 hours after normothermic machine perfusion in extended criteria donors livers.
Time frame: 2.5 hours
Bile gas lactate 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas lactate 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Markers of injury, inflammation 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Markers of injury, inflammation 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas Glucose 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas Glucose 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas pH 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas pH 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas O2 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas O2 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas CO2 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas CO2 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas HCO3 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas HCO3 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas SO2 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas SO2 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas Hemoglobin 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas Hemoglobin 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas Na 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas Na 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas K 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas K 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas Ca 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas Ca 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Bile gas BE 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion
Bile gas BE 2 hours after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Markers of injury, inflammation 1 hour after controlled oxygenated rewarming machine perfusion
Markers of injury, inflammation 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Bile gas lactate 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas lactate 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas Glucose 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas Glucose 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas pH 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas pH 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Markers of injury, inflammation 1 hour after controlled oxygenated rewarming machine perfusion
Markers of injury, inflammation 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas O2 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas O2 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas CO2 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas CO2 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas HCO3 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas HCO3 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas BE 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas BE 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas SO2 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas SO2 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas hemoglobin 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas hemoglobin 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas Na 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas Na 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas K 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas K 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Bile gas Ca 1 hour after controlled oxygenated rewarming machine perfusion
Bile gas Ca 1 hour after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hours
Perfusate gas Ca - during machine perfusion
Perfusate gas Ca during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas pH - during machine perfusion
Perfusate gas pH during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas SO2 - during machine perfusion
Perfusate gas SO2 during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas O2 - during machine perfusion
Perfusate gas O2 during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas CO2 - during machine perfusion
Perfusate gas CO2 during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas K - during machine perfusion
Perfusate gas K during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas Na - during machine perfusion
Perfusate gas Na during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas BE - during machine perfusion
Perfusate gas BE during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas HCO3 - during machine perfusion
Perfusate gas HCO3 during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas hemoglobin - during machine perfusion
Perfusate gas hemoglobin during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas lactate - during machine perfusion
Perfusate gas lactate during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Perfusate gas glucose - during machine perfusion
Perfusate gas glucose during machine perfusion in extended criteria donors livers.
Time frame: 24 hours
Postoperative complications
According to Clavien Dindo Classification
Time frame: 12 months
Length of stay
Length of initial ICU and initial hospital stay is determined in days of admission following liver transplantation.
Time frame: 4 months
Machine flow during controlled oxygenated rewarming
Machine flow (millilitre/hour)
Time frame: 1 hour
Machine flow during Dual-end-ischemic Hypothermic Oxygenated Perfusion
Machine flow (millilitre/hour)
Time frame: 1 hour
Machine pressure during controlled oxygenated rewarming
Machine pressure ((millimetre Hg))
Time frame: 1 hour
Machine pressure during Dual-end-ischemic Hypothermic Oxygenated Perfusion
Machine pressure ((millimetre Hg))
Time frame: 1 hour
Machine Fraction of oxygen during controlled oxygenated rewarming
Machine fraction of oxygen (%)
Time frame: 1 hour
Machine Fraction of oxygen Dual-end-ischemic Hypothermic Oxygenated Perfusion
Machine fraction of oxygen (%)
Time frame: 1 hour
Machine Resistance during controlled oxygenated rewarming
Machine Resistance (millilitre/minute/millimetre Hg)
Time frame: 1 hour
Machine Resistance during Dual-end-ischemic Hypothermic Oxygenated Perfusion
Machine Resistance (millilitre/minute/millimetre Hg)
Time frame: 1 hour
Machine flow during Dual-end-ischemic Hypothermic Oxygenated Perfusion
Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion flow (millilitre/hour)
Time frame: 2 hours
Machine flow during normothermic machine perfusion
Machine flow during normothermic machine perfusion (millilitre/hour)
Time frame: 4 hours
Machine flow during normothermic machine perfusion
Machine flow during normothermic machine perfusion (millilitre/hour)
Time frame: 2.5 hours
Machine pressure during normothermic machine perfusion
Machine pressure (millimetre Hg)
Time frame: 4 hours
Machine pressure during normothermic machine perfusion
Machine pressure (millimetre Hg)
Time frame: 2.5 hours
Machine Fraction of oxygen during normothermic machine perfusion
Machine fraction of oxygen (%)
Time frame: 4 hours
Machine Fraction of oxygen during normothermic machine perfusion
Machine fraction of oxygen (%)
Time frame: 2.5 hours
Machine Resistance during normothermic machine perfusion
Machine Resistance (millilitre/minute/millimetre Hg)
Time frame: 4 hours
Machine Resistance during normothermic machine perfusion
Machine Resistance (millilitre/minute/millimetre Hg)
Time frame: 2.5 hours
Total preservation time
Total preservation time (machine perfusion time)
Time frame: 48 hours
Alanine amino transferase in perfusate during machine perfusion
Alanine amino transferase in perfusate during machine perfusion (U/Litre)
Time frame: 24 hours
Aspartate amino transferase in perfusate during machine perfusion
Aspartate amino transferase in perfusate during machine perfusion (U/Litre)
Time frame: 24 hours
Alkaline phosphatase in perfusate during machine perfusion
Aspartate amino transferase in perfusate during machine perfusion (U/Litre)
Time frame: 24 hours
Gamma-glutamyl transferase in perfusate during machine perfusion
Gamma-glutamyl transferase in perfusate during machine perfusion (U/Litre)
Time frame: 24 hours
Urea in perfusate during machine perfusion
Urea in perfusate during machine perfusion (millimol/Litre)
Time frame: 24 hours
Bilirubin in perfusate during machine perfusion
Bilirubin in perfusate during machine perfusion (micromol/Litre)
Time frame: 24 hours
Not yet decided types of cytokines/interleukins, endothelium markers, miRNA's in perfusate during machine perfusion
Different cytokines/interleukins, endothelium markers, miRNA's in perfusate during machine perfusion
Time frame: 24 hours
Not yet decided types of cytokines/interleukins, endothelium markers, miRNA's in liver tissue during machine perfusion
Different cytokines/interleukins, endothelium markers, miRNA's in liver tissue during machine perfusion
Time frame: 24 hours
Not yet decided types of cytokines/interleukins, endothelium markers, miRNA's in bile during machine perfusion
Different cytokines/interleukins, endothelium markers, miRNA's in bile during machine perfusion
Time frame: 24 hours
Histopathology - liver tissue. Mitochondrial function
Histological analysis of parenchymal biopsies which are assessed for mitochondrial function.
Time frame: During machine perfusion - 24 hours
Histopathology - bile duct. Mitochondrial function
Histological analysis of bile duct biopsies which are assessed for mitochondrial function.
Time frame: During machine perfusion - 24 hours
Histopathology - liver tissue. Perfusion/reperfusion injury
Histological analysis of parenchymal biopsies which are assessed for perfusion/reperfusion injury
Time frame: During machine perfusion - 24 hours
Histopathology - bile duct. Perfusion/reperfusion injury
Histological analysis of bile duct biopsies which are assessed for perfusion/reperfusion injury
Time frame: During machine perfusion - 24 hours
Histopathology - liver tissue. Cell death
Histological analysis of parenchymal biopsies which are assessed for cell death
Time frame: During machine perfusion - 24 hours
Histopathology - bile duct. Cell death
Histological analysis of bile duct biopsies which are assessed for cell death
Time frame: During machine perfusion - 24 hours
Histopathology - liver tissue. Inflammation
Histological analysis of parenchymal biopsies which are assessed for inflammation
Time frame: During machine perfusion - 24 hours
Histopathology - bile duct. Inflammation
Histological analysis of bile duct biopsies which are assessed for inflammation
Time frame: During machine perfusion - 24 hours
Histopathology - liver tissue. Metabolomics
Histological analysis of parenchymal biopsies which are assessed for metabolomics
Time frame: During machine perfusion - 24 hours
Histopathology - bileduct. Metabolomics
Histological analysis of bile duct biopsies which are assessed for metabolomics
Time frame: During machine perfusion - 24 hours
Histopathology - liver tissue. Lipidomics
Histological analysis of parenchymal biopsies which are assessed for lipidomics
Time frame: During machine perfusion - 24 hours
Histopathology - bile duct. Lipidomics
Histological analysis of bile duct biopsies which are assessed for lipidomics
Time frame: During machine perfusion - 24 hours
Histopathology - liver tissue. RNA sequencing
Histological analysis of parenchymal biopsies which are assessed for RNA sequencing
Time frame: During machine perfusion - 24 hours
Histopathology - bile duct. RNA sequencing
Histological analysis of bile duct biopsies which are assessed for RNA sequencing
Time frame: During machine perfusion - 24 hours
Histopathology - liver tissue. Endothelium properties
Histological analysis of parenchymal biopsies which are assessed for endothelium properties
Time frame: During machine perfusion - 24 hours
Histopathology - bile duct. Endothelium properties
Histological analysis of bile duct biopsies which are assessed for endothelium properties
Time frame: During machine perfusion - 24 hours
International normalized ratio on postoperative day 7
Blood sample from recipient: International normalized ratio on postoperative day 7
Time frame: 7 postoperative day
Alanine amino transferase postoperative day 0
Alanine amino transferase postoperative day 0 in the donated recipients (U/Litre)
Time frame: Postoperative day 0
Aspartate amino transferase postoperative day 0
Aspartate amino transferase postoperative day 0 in the donated recipients (U/Litre)
Time frame: Postoperative day 0
Alkaline phosphatase postoperative day 0
Alkaline phosphatase postoperative day 0 in the donated recipients (U/Litre)
Time frame: Postoperative day 0
Gamma-glutamyl transferase postoperative day 0
Gamma-glutamyl transferase postoperative day 0 in the donated recipients (U/Litre)
Time frame: Postoperative day 0
Urea postoperative day 0
Urea postoperative day 0 in the donated recipients (millimol/Litre)
Time frame: Postoperative day 0
Bilirubin postoperative day 0
Bilirubin postoperative day 0 in the donated recipients (micromol/Litre)
Time frame: Postoperative day 0
Bilirubin postoperative day 1
Bilirubin postoperative day 1 in the donated recipients (micromol/Litre)
Time frame: Postoperative day 1
Bilirubin postoperative day 2
Bilirubin postoperative day 2 in the donated recipients (micromol/Litre)
Time frame: Postoperative day 2
Bilirubin postoperative day 3
Bilirubin postoperative day 3 in the donated recipients (micromol/Litre)
Time frame: Postoperative day 3
Bilirubin postoperative day 4
Bilirubin postoperative day 4 in the donated recipients (micromol/Litre)
Time frame: Postoperative day 4
Bilirubin postoperative day 5
Bilirubin postoperative day 5 in the donated recipients (micromol/Litre)
Time frame: Postoperative day 5
Bilirubin postoperative day 6
Bilirubin postoperative day 6 in the donated recipients (micromol/Litre)
Time frame: Postoperative day 6
Bilirubin postoperative day 7
Bilirubin postoperative day 7 in the donated recipients (micromol/Litre)
Time frame: Postoperative day 7
Urea postoperative day 1
Urea postoperative day 1 in the donated recipients (millimol/Litre)
Time frame: Postoperative day 1
Urea postoperative day 2
Urea postoperative day 2 in the donated recipients (millimol/Litre)
Time frame: Postoperative day 2
Urea postoperative day 3
Urea postoperative day 3 in the donated recipients (millimol/Litre)
Time frame: Postoperative day 3
Urea postoperative day 4
Urea postoperative day 4 in the donated recipients (millimol/Litre)
Time frame: Postoperative day 4
Urea postoperative day 5
Urea postoperative day 5 in the donated recipients (millimol/Litre)
Time frame: Postoperative day 5
Urea postoperative day 6
Urea postoperative day 6 in the donated recipients (millimol/Litre)
Time frame: Postoperative day 6
Urea postoperative day 7
Urea postoperative day 7 in the donated recipients (millimol/Litre)
Time frame: Postoperative day 7
Aspartate amino transferase postoperative day 1
Aspartate amino transferase postoperative day 1 in the donated recipients (U/Litre)
Time frame: Postoperative day 1
Alkaline phosphatase postoperative day 1
Alkaline phosphatase postoperative day 1 in the donated recipients (U/Litre)
Time frame: Postoperative day 1
Gamma-glutamyl transferase postoperative day 1
Gamma-glutamyl transferase postoperative day 1 in the donated recipients (U/Litre)
Time frame: Postoperative day 1
Alanine amino transferase postoperative day 2
Alanine amino transferase postoperative day 2 in the donated recipients (U/Litre)
Time frame: Postoperative day 2
Aspartate amino transferase postoperative day 2
Aspartate amino transferase postoperative day 2 in the donated recipients (U/Litre)
Time frame: Postoperative day 2
Alkaline phosphatase postoperative day 2
Alkaline phosphatase postoperative day 2 in the donated recipients (U/Litre)
Time frame: Postoperative day 2
Gamma-glutamyl transferase postoperative day 2
Gamma-glutamyl transferase postoperative day 2 in the donated recipients (U/Litre)
Time frame: Postoperative day 2
Alanine amino transferase postoperative day 3
Alanine amino transferase postoperative day 3 in the donated recipients (U/Litre)
Time frame: Postoperative day 3
Aspartate amino transferase postoperative day 3
Aspartate amino transferase postoperative day 3 in the donated recipients (U/Litre)
Time frame: Postoperative day 3
Alkaline phosphatase postoperative day 3
Alkaline phosphatase postoperative day 3 in the donated recipients (U/Litre)
Time frame: Postoperative day 3
Gamma-glutamyl transferase postoperative day 3
Gamma-glutamyl transferase postoperative day 3 in the donated recipients (U/Litre)
Time frame: Postoperative day 3
Alanine amino transferase postoperative day 4
Alanine amino transferase postoperative day 4 in the donated recipients (U/Litre)
Time frame: Postoperative day 4
Aspartate amino transferase postoperative day 4
Aspartate amino transferase postoperative day 4 in the donated recipients (U/Litre)
Time frame: Postoperative day 4
Alkaline phosphatase postoperative day 4
Alkaline phosphatase postoperative day 4 in the donated recipients (U/Litre)
Time frame: Postoperative day 4
Gamma-glutamyl transferase postoperative day 4
Gamma-glutamyl transferase postoperative day 4 in the donated recipients (U/Litre)
Time frame: Postoperative day 4
Alanine amino transferase postoperative day 5
Alanine amino transferase postoperative day 5 in the donated recipients (U/Litre)
Time frame: Postoperative day 5
Aspartate amino transferase postoperative day 5
Aspartate amino transferase postoperative day 5 in the donated recipients (U/Litre)
Time frame: Postoperative day 5
Alkaline phosphatase postoperative day 5
Alkaline phosphatase postoperative day 5 in the donated recipients (U/Litre)
Time frame: Postoperative day 5
Gamma-glutamyl transferase postoperative day 5
Gamma-glutamyl transferase postoperative day 5 in the donated recipients (U/Litre)
Time frame: Postoperative day 5
Alanine amino transferase postoperative day 6
Alanine amino transferase postoperative day 6 in the donated recipients (U/Litre)
Time frame: Postoperative day 6
Aspartate amino transferase postoperative day 6
Aspartate amino transferase postoperative day 6 in the donated recipients (U/Litre)
Time frame: Postoperative day 6
Alkaline phosphatase postoperative day 6
Aspartate amino transferase postoperative day 6 in the donated recipients (U/Litre)
Time frame: Postoperative day 6
Gamma-glutamyl transferase postoperative day 6
Gamma-glutamyl transferase postoperative day 6 in the donated recipients (U/Litre)
Time frame: Postoperative day 6
Alanine amino transferase postoperative day 7
Alanine amino transferase postoperative day 7 in the donated recipients (U/Litre)
Time frame: Postoperative day 7
Aspartate amino transferase postoperative day 7
Aspartate amino transferase postoperative day 7 in the donated recipients (U/Litre)
Time frame: Postoperative day 7
Alkaline phosphatase postoperative day 7
Alkaline phosphatase postoperative day 7 in the donated recipients (U/Litre)
Time frame: Postoperative day 7
Gamma-glutamyl transferase postoperative day 7
Gamma-glutamyl transferase postoperative day 7 in the donated recipients (U/Litre)
Time frame: Postoperative day 7
Alanine amino transferase postoperative month 1
Alanine amino transferase postoperative month 1 in the donated recipients (U/Litre)
Time frame: 1 month after surgery
Aspartate amino transferase postoperative month 1
Aspartate amino transferase postoperative month 1 in the donated recipients (U/Litre)
Time frame: 1 month after surgery
Alkaline phosphatase postoperative month 1
Alkaline phosphatase postoperative month 1 in the donated recipients (U/Litre)
Time frame: 1 month after surgery
Gamma-glutamyl transferase postoperative month 1
Gamma-glutamyl transferase postoperative month 1 in the donated recipients (U/Litre)
Time frame: 1 month after surgery
Urea postoperative month 1
Urea postoperative month 1 in the donated recipients (millimol/Litre)
Time frame: 1 month after surgery
Bilirubin postoperative month 1
Bilirubin postoperative month 1 in the donated recipients (micromol/Litre)
Time frame: 1 month after surgery
Bilirubin postoperative month 2
Bilirubin postoperative month 2 in the donated recipients (micromol/Litre)
Time frame: 2 month after surgery
Bilirubin postoperative month 3
Bilirubin postoperative month 3 in the donated recipients (micromol/Litre)
Time frame: 3 month after surgery
Bilirubin postoperative month 4
Bilirubin postoperative month 4 in the donated recipients (micromol/Litre)
Time frame: 4 month after surgery
Bilirubin postoperative month 5
Bilirubin postoperative month 5 in the donated recipients (micromol/Litre)
Time frame: 5 month after surgery
Bilirubin postoperative month 6
Bilirubin postoperative month 6 in the donated recipients (micromol/Litre)
Time frame: 6 month after surgery
Bilirubin postoperative month 7
Bilirubin postoperative month 7 in the donated recipients (micromol/Litre)
Time frame: 7 month after surgery
Bilirubin postoperative month 8
Bilirubin postoperative month 8 in the donated recipients (micromol/Litre)
Time frame: 8 month after surgery
Bilirubin postoperative month 9
Bilirubin postoperative month 9 in the donated recipients (micromol/Litre)
Time frame: 9 month after surgery
Bilirubin postoperative month 10
Bilirubin postoperative month 10 in the donated recipients (micromol/Litre)
Time frame: 10 month after surgery
Bilirubin postoperative month 11
Bilirubin postoperative month 11 in the donated recipients (micromol/Litre)
Time frame: 11 month after surgery
Bilirubin postoperative month 12
Bilirubin postoperative month 12 in the donated recipients (micromol/Litre)
Time frame: 12 month after surgery
Urea postoperative month 2
Urea postoperative month 2 in the donated recipients (millimol/Litre)
Time frame: 2 month after surgery
Urea postoperative month 3
Urea postoperative month 3 in the donated recipients (millimol/Litre)
Time frame: 3 month after surgery
Urea postoperative month 4
Urea postoperative month 4 in the donated recipients (millimol/Litre)
Time frame: 4 month after surgery
Urea postoperative month 5
Urea postoperative month 5 in the donated recipients (millimol/Litre)
Time frame: 5 month after surgery
Urea postoperative month 6
Urea postoperative month 6 in the donated recipients (millimol/Litre)
Time frame: 6 month after surgery
Urea postoperative month 7
Urea postoperative month 7 in the donated recipients (millimol/Litre)
Time frame: 7 month after surgery
Urea postoperative month 8
Urea postoperative month 8 in the donated recipients (millimol/Litre)
Time frame: 8 month after surgery
Urea postoperative month 9
Urea postoperative month 9 in the donated recipients (millimol/Litre)
Time frame: 9 month after surgery
Urea postoperative month 10
Urea postoperative month 10 in the donated recipients (millimol/Litre)
Time frame: 10 month after surgery
Urea postoperative month 11
Urea postoperative month 11 in the donated recipients (millimol/Litre)
Time frame: 11 month after surgery
Urea postoperative month 12
Urea postoperative month 12 in the donated recipients (millimol/Litre)
Time frame: 12 month after surgery
Alanine amino transferase postoperative month 2
Alanine amino transferase postoperative month 2 in the donated recipients (U/Litre)
Time frame: 2 month after surgery
Aspartate amino transferase postoperative month 2
Aspartate amino transferase postoperative month 2 in the donated recipients (U/Litre)
Time frame: 2 month after surgery
Alkaline phosphatase postoperative month 2
Alkaline phosphatase postoperative month 2 in the donated recipients (U/Litre)
Time frame: 2 month after surgery
Gamma-glutamyl transferase postoperative month 2
Gamma-glutamyl transferase postoperative month 2 in the donated recipients (U/Litre)
Time frame: 2 month after surgery
Alanine amino transferase postoperative month 3
Alanine amino transferase postoperative month 3 in the donated recipients (U/Litre)
Time frame: 3 month after surgery
Aspartate amino transferase postoperative month 3
Aspartate amino transferase postoperative month 3 in the donated recipients (U/Litre)
Time frame: 3 month after surgery
Alkaline phosphatase postoperative month 3
Alkaline phosphatase postoperative month 3 in the donated recipients (U/Litre)
Time frame: 3 month after surgery
Gamma-glutamyl transferase postoperative month 3
Gamma-glutamyl transferase postoperative month 3 in the donated recipients (U/Litre)
Time frame: 3 month after surgery
Alanine amino transferase postoperative month 4
Alanine amino transferase postoperative month 4 in the donated recipients (U/Litre)
Time frame: 4 month after surgery
Aspartate amino transferase postoperative month 4
Aspartate amino transferase postoperative month 4 in the donated recipients (U/Litre)
Time frame: 4 month after surgery
Alkaline phosphatase postoperative month 4
Alkaline phosphatase postoperative month 4 in the donated recipients (U/Litre)
Time frame: 4 month after surgery
Gamma-glutamyl transferase postoperative month 4
Gamma-glutamyl transferase postoperative month 4 in the donated recipients (U/Litre)
Time frame: 4 month after surgery
Alanine amino transferase postoperative month 5
Alanine amino transferase postoperative month 5 in the donated recipients (U/Litre)
Time frame: 5 month after surgery
Aspartate amino transferase postoperative month 5
Aspartate amino transferase postoperative month 5 in the donated recipients (U/Litre)
Time frame: 5 month after surgery
Alkaline phosphatase postoperative month 5
Alkaline phosphatase postoperative month 5 in the donated recipients (U/Litre)
Time frame: 5 month after surgery
Gamma-glutamyl transferase postoperative month 5
Gamma-glutamyl transferase postoperative month 5 in the donated recipients (U/Litre)
Time frame: 5 month after surgery
Alanine amino transferase postoperative month 6
Alanine amino transferase postoperative month 6 in the donated recipients (U/Litre)
Time frame: 6 month after surgery
Aspartate amino transferase postoperative month 6
Aspartate amino transferase postoperative month 6 in the donated recipients (U/Litre)
Time frame: 6 month after surgery
Alkaline phosphatase postoperative month 6
Alkaline phosphatase postoperative month 6 in the donated recipients (U/Litre)
Time frame: 6 month after surgery
Gamma-glutamyl transferase postoperative month 6
Gamma-glutamyl transferase postoperative month 6 in the donated recipients (U/Litre)
Time frame: 6 month after surgery
Alanine amino transferase postoperative month 7
Alanine amino transferase postoperative month 7 in the donated recipients (U/Litre)
Time frame: 7 month after surgery
Aspartate amino transferase postoperative month 7
Aspartate amino transferase postoperative month 7 in the donated recipients (U/Litre)
Time frame: 7 month after surgery
Alkaline phosphatase postoperative month 7
Alkaline phosphatase postoperative month 7 in the donated recipients (U/Litre)
Time frame: 7 month after surgery
Gamma-glutamyl transferase postoperative month 7
Gamma-glutamyl transferase postoperative month 7 in the donated recipients (U/Litre)
Time frame: 7 month after surgery
Alanine amino transferase postoperative month 8
Alanine amino transferase postoperative month 8 in the donated recipients (U/Litre)
Time frame: 8 month after surgery
Aspartate amino transferase postoperative month 8
Aspartate amino transferase postoperative month 8 in the donated recipients (U/Litre)
Time frame: 8 month after surgery
Alkaline phosphatase postoperative month 8
Alkaline phosphatase postoperative month 8 in the donated recipients (U/Litre)
Time frame: 8 month after surgery
Gamma-glutamyl transferase postoperative month 8
Gamma-glutamyl transferase postoperative month 8 in the donated recipients (U/Litre)
Time frame: 8 month after surgery
Alanine amino transferase postoperative month 9
Alanine amino transferase postoperative month 9 in the donated recipients (U/Litre)
Time frame: 9 month after surgery
Aspartate amino transferase postoperative month 9
Aspartate amino transferase postoperative month 9 in the donated recipients (U/Litre)
Time frame: 9 month after surgery
Alkaline phosphatase postoperative month 9
Alkaline phosphatase postoperative month 9 in the donated recipients (U/Litre)
Time frame: 9 month after surgery
Gamma-glutamyl transferase postoperative month 9
Gamma-glutamyl transferase postoperative month 9 in the donated recipients (U/Litre)
Time frame: 9 month after surgery
Alanine amino transferase postoperative month 10
Alanine amino transferase postoperative month 10 in the donated recipients (U/Litre)
Time frame: 10 month after surgery
Aspartate amino transferase postoperative month 10
Aspartate amino transferase postoperative month 10 in the donated recipients (U/Litre)
Time frame: 10 month after surgery
Alkaline phosphatase postoperative month 10
Alkaline phosphatase postoperative month 10 in the donated recipients (U/Litre)
Time frame: 10 month after surgery
Gamma-glutamyl transferase postoperative month 10
Gamma-glutamyl transferase postoperative month 10 in the donated recipients (U/Litre)
Time frame: 10 month after surgery
Alanine amino transferase postoperative month 11
Alanine amino transferase postoperative month 11 in the donated recipients (U/Litre)
Time frame: 11 month after surgery
Aspartate amino transferase postoperative month 11
Aspartate amino transferase postoperative month 11 in the donated recipients (U/Litre)
Time frame: 11 month after surgery
Alkaline phosphatase postoperative month 11
Alkaline phosphatase postoperative month 11 in the donated recipients (U/Litre)
Time frame: 11 month after surgery
Gamma-glutamyl transferase postoperative month 11
Gamma-glutamyl transferase postoperative month 11 in the donated recipients (U/Litre)
Time frame: 11 month after surgery
Alanine amino transferase postoperative month 12
Alanine amino transferase postoperative month 12 in the donated recipients (U/Litre)
Time frame: 12 month after surgery
Aspartate amino transferase postoperative month 12
Aspartate amino transferase postoperative month 12 in the donated recipients (U/Litre)
Time frame: 12 month after surgery
Alkaline phosphatase postoperative month 12
Alkaline phosphatase postoperative month 12 in the donated recipients (U/Litre)
Time frame: 12 month after surgery
Gamma-glutamyl transferase postoperative month 12
Gamma-glutamyl transferase postoperative month 12 in the donated recipients (U/Litre)
Time frame: 12 month after surgery
Postoperative microdialysis levels of lactate
Postoperative microdialysis levels of lactate in transplanted livers the first 14 postoperative days
Time frame: 14 days
Postoperative microdialysis levels of pyruvate
Postoperative microdialysis levels of pyruvate in transplanted livers the first 14 postoperative days
Time frame: 14 days
Postoperative microdialysis levels of glycerol
Postoperative microdialysis levels of glycerol in transplanted livers the first 14 postoperative days
Time frame: 14 days
Postoperative microdialysis levels of glucose
Postoperative microdialysis levels of glucose in transplanted livers the first 14 postoperative days
Time frame: 14 days
Systolic blood pressure
Systolic blood pressure (millimeter Hg)
Time frame: From 5 min before reperfusion, up to 20 minutes after reperfusion
Diastolic blood pressure
Diastolic blood pressure (millimeter Hg)
Time frame: From 5 min before reperfusion, up to 20 minutes after reperfusion
Heart rate
Heart rate (Beats/minute)
Time frame: From 5 min before reperfusion, up to 20 minutes after reperfusion
Vasopressor dosage
Vasopressor dosage (microgram/kilogram/minutes)
Time frame: From 5 min before reperfusion, up to 20 minutes after reperfusion
Patient survival
Patient survival (days)
Time frame: Through study completion, an average of 12 months
Graft survival
Graft (censored and uncensored for patient death) survival (days)
Time frame: Through study completion, an average of 12 months
Number of deaths
Number of deaths first 7 days after transplantation
Time frame: 7 days after transplantation
Number of retransplantation
Number of retransplantation first 7 days after transplantation
Time frame: 7 days after transplantation
Incidens of new onset diabetes after transplantation
New onset diabetes after transplantation after transplantation. New onset diabetes after transplantation is defined according to the World Health Organization criteria (Report 2003 S5-20).
Time frame: Through study completion, an average of 12 months
Estimated glomerular filtration rate - Renal function
Estimated glomerular filtration rate according to the 4-variable Modification of Diet in Renal Disease equation.
Time frame: Through study completion, an average of 12 months
The incidence of symptomatic Non-anastomotic Biliary Strictures
The incidence of symptomatic non-anastomotic biliary strictures
Time frame: 6 months
The incidence of asymptomatic Non-anastomotic Biliary Strictures
The incidence of asymptomatic non-anastomotic biliary strictures
Time frame: 6 months
The incidence of asymptomatic Non-anastomotic Biliary Strictures
The incidence of asymptomatic non-anastomotic biliary strictures
Time frame: 12 months
The incidence of symptomatic Non-anastomotic Biliary Strictures
The incidence of symptomatic non-anastomotic biliary strictures
Time frame: 12 months
The severity of Non-anastomotic Biliary Strictures
The severity of Non-anastomotic Biliary Strictures based on a scoring system described by Buis et. al (mild, moderate, or severe)
Time frame: Through study completion, an average of 12 months
The location of Non-anastomotic Biliary Strictures
The location of Non-anastomotic Biliary Strictures is assessed by images of the magnetic resonance cholangiopancreatography
Time frame: Through study completion, an average of 12 months
The incidence of infections
The incidence of infections leading to administration of antibiotics/antifungal/antiviral treatment
Time frame: Through study completion, an average of 12 months
Lactate level in microdialysate from liver tissue - 4 hours
Microdialysis lactate level in donor liver tissue after 4 hours of normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Pyruvate level in microdialysate from hilar plate after normothermic machine perfusion - 4 hours
Pyruvate level in microdialysate from hilar plate after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Glycerol level in microdialysate from hilar plate after normothermic machine perfusion - 4 hours
Glycerol level in microdialysate from hilar plate after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Glucose level in microdialysate from hilar plate after normothermic machine perfusion - 4 hours
Glucose level in microdialysate from hilar plate after normothermic machine perfusion in extended criteria donors livers.
Time frame: 4 hours
Glycerol level in microdialysate from hilar plate after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Glycerol level in microdialysate from hilar plate after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Lactate level in microdialysate from hilar plate after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion - 2 hours
Lactate level in microdialysate from hilar plate after Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hours
Glucose level in microdialysate from hilar plate after controlled oxygenated rewarming machine perfusion - 1 hour
Glucose level in microdialysate from hilar plate after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Pyruvate level in microdialysate from hilar plate after controlled oxygenated rewarming machine perfusion - 1 hour
Pyruvate level in microdialysate from hilar plate after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Glycerol level in microdialysate from hilar plate after controlled oxygenated rewarming machine perfusion - 1 hour
Glycerol level in microdialysate from hilar plate after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Lactate level in microdialysate from hilar plate after controlled oxygenated rewarming machine perfusion - 1 hour
Lactate level in microdialysate from hilar plate after controlled oxygenated rewarming machine perfusion in extended criteria donors livers.
Time frame: 1 hour
Glucose level in microdialysate from hilar plate after of Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers - 2 hours
Glucose level in microdialysate from hilar plate after of Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hour
Pyruvate level in microdialysate from hilar plate after of Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers - 2 hours
Pyruvate level in microdialysate from hilar plate after of Dual-end-ischemic Hypothermic Oxygenated Perfusion machine perfusion in extended criteria donors livers.
Time frame: 2 hour
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