This study is an investigator-initiated, randomised, double-blind, placebo-controlled, cross-over human trial investigating the effect of DHA-NAT (C22:6 N-acyl taurine, an endogenous metabolite derived from the omega-3 fatty acid, docosahexaenoic acid) on postprandial plasma triglyceride levels following a high-fat meal.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
DOUBLE
Enrollment
20
Center for Clinical Metabolic Research, Herlev-Gentofte Hospital
Hellerup, Denmark
Plasma triglyceride
Change in incremental area-under-curve plasma triglyceride
Time frame: 0-240 minutes
Plasma GLP-1
Plasma GLP-1 profile
Time frame: 0-240 minutes
Plasma N-acyl taurine species
Profile of plasma n-acyl taurine species
Time frame: 0-240 minutes
Plasma ApoB48
Plasma ApoB48 profile
Time frame: 0-240 minutes
Satiety, hunger and appetite
Development during study day recorded by visual analogue scale (VAS), 0-10 cm where 0 = not in agreement with statement and 10 = in agreement with statement.
Time frame: 0-240 minutes
Food intake (g)
Grams consumed of a standard ad libitum meal
Time frame: t=240 minutes
Food intake (kcal)
kcal consumed of a standard ad libitum meal
Time frame: t=240 minutes
Plasma triglyceride (TG) distribution
total TG, and TG in HDL, LDL and VLDL cholesterol, remnant TG
Time frame: 0-240 minutes
Plasma free fatty acid species
Total fatty acids profile
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Time frame: 0-240 minutes
Plasma cholesterol
Total cholesterol, HDL, LDL, VLDL, remnant cholesterol
Time frame: 0-240 minutes
Plasma level of glucose regulating hormones
Insulin, glucagon and gastric inhibitory polypeptide
Time frame: 0-240 minutes
Plasma amino acids
Total amino acids profile
Time frame: 0-240 minutes
Plasma glucose
Plasma glucose profile
Time frame: 0-240 minutes
Plasma bile acid species
Plasma bile acid profile
Time frame: 0-240 minutes
Gall bladder emptying: Cholecystokinin
Cholecystokinin profile
Time frame: 0-240 minutes
Gall bladder emptying: Ultrasonography
Difference in volume from most to least full
Time frame: 0-240 minutes
Gastric emptying: Paracetamol
Plasma acetaminphen profile
Time frame: 0-240 minutes
Stool quality
Self-assessment of stool quality by the Bristol Stool Scale (1-7, where 1 = most solid and 7 = least solid)
Time frame: Up to 24 hours from ingestion of test meal or until first bowel movement if later than 24 hours.