Defining Circadian Metabolism in Non-alcoholic Fatty Liver Disease

Inclusion Criteria: * Male or female * BMI 25-50 kg/m2 * Age 18-75 years Exclusion Criteria: * A diagnosis of type 1 diabetes * Type 2 diabetes controlled with medications other than metformin (within last 3 months) * Shift working patterns in last 3-months or if likely to work shifts during study period. * Current or recent (\<3-months) use of weight loss medications * Current or recent use of oral contraceptive pill or hormone replacement therapy (within last 3-months) * Pregnancy * A blood haemoglobin \<120mg/dL * History of alcoholism or a greater than recommended alcohol intake (Recommendations \> 21 units on average per week for men and \> 14 units on average per week for women) * Haemorrhagic disorders * Treatment with anticoagulant agents * Other co-morbidities that in the eyes of the investigators may affect data collection * Any medical condition in the opinion of the investigator that might impact upon safety or validity of the results * Transient elastography (TE) controlled attenuation parameter (CAP) of between 216-305 dB/m