The Potential Association of Long Non-coding RNA (NEAT1 and MALAT1) in the Process of Epithelial-mesenchymal Transition in Periodontitis

Ali A Abdulkareem48 enrolled

Overview

Periodontitis is a polymicrobial, inflammatory condition affecting tooth-supporting tissues. It is characterized by a progressive loss of epithelial attachment and resorption of alveolar bone, which can lead to tooth loss. Epithelial-mesenchymal transition (EMT) has been proposed as a reversible process that shifts the cell phenotype from epithelial to mesenchymal-like and may be involved in the process of periodontal inflammation either completely or partially. Long non-coding RNAs (lncRNAs) are a large class of regulatory transcripts longer than 200 nucleotides lacking evident protein-coding potential. Studies have shown that lncRNA dysregulation plays key roles in human diseases, including cancer, by modulating the epithelial-mesenchymal transition (EMT). The involvement of lncRNAs markers in the pathogenesis of periodontal disease induced EMT has not been studied thoroughly and have created a gap in the knowledge.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Periodontal Disease, AVDC Stage 3and 4 Epithelial Mesenchymal Transition Long Noncoding RNA

Interventions

Eligibility

Sex: ALLHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Patients with no history of systemic disease e.g., diabetes. * Both males and females will be included in the study. * Control group: patients fit the case definition of gingival health2, and confirmed by Histopathological inflammatory cell infiltration analysis. * Periodontitis group: patients fit the case definition of periodontal disease referred for periodontal flap surgery (modified Widman's flap). * Patients radiographically confirmed with infrabony pocket eligible for periodontal flap surgery. Exclusion Criteria: * Patients not willing to participate or consent. * Patient taking medications that affect or modify immunity (e.g., corticosteroid), for the last 3 months. * Patient taking NSAIDs for the last 3 months. * Active smoker.

Outcomes

Primary Outcomes

determining the relative expression of lncRNAs (NEAT1 and MALAT1) in healthy and diseased periodontal tissue.

reverse transcription real time polymerase chain reaction

Time frame: one year

Secondary Outcomes

determine the expression of EMT-related genes (E-cadherin, Vimentin, β-catenin, and Snail1), in healthy and diseased tissues, and their correlation with lncRNAs and clinical parameters.