Organoid Model to unravel Klinefelter Syndrome infertility Klinefelter Syndrome (KS) is characterized by the presence of an extra chromosome X in male (47,XXY), it is the most frequent genetic cause of azoospermia in adult men. The investigators will isolate and expand spermatogonial cells from KS patients, then using an organoid model investigators will compare the behavior of these Spermatogonia from KS patients when interacting with four combinations of somatic cell types incorporated in the Extra Cellular Matrix hydrogel.
Men with Klinefelter Syndrome candidate for TESE or microTESE (testicular sperm extraction) for azoospermia. One extra testicular biopsy (1-2 mm) will be cryopreserved and used later for the cells isolation and culture.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
20
Testicular biopsy is a clinical procedure for extraction of testicular tissue
Cliniques Universitaires St Luc
Brussels, Belgium
RECRUITINGKlinefelter's infertility physiopathology
Testicular biopsies will be digested using two steps enzymatic protocol. Cell suspensions will be in vitro cultured during 2 months. The following analyses will be performed: cells counts, cells characterisation by immunofluorescence and RTqPCR specific for the cell type, cells karyotypes.
Time frame: Up to 60 days with intermediate time analyses at the 5th and 10 th passage of cultures.
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