Cortisol is an essential for life hormone secreted in a pulsatile pattern on a diurnal rhythm. Given the complexity of cortisol secretion on a circadian rhythm with pulsations, current methods of cortisol measurement have limitations. Therefore, a non-invasive and ambulatory method would be useful to measure cortisol levels in real-time. The main aim of the study is to compare cortisol levels across biological fluids (sweat, saliva, interstitial fluid, and blood) in order to validate in the long term a continuous and non-invasive cortisol measurement device (currently under development).
Study Type
OBSERVATIONAL
Enrollment
12
24 hours sampling of 4 biological fluids: * blood: sampling every 20 minutes (through a venous catheter) * ISF: automated sampling every 20 minutes (microdialysis technique connected to U-Rhythm device) * Sweat : collection of 1 to 3 samples at 4 differents timepoints * Saliva : sampling every hour (except during the night)
24 hours sampling in an ambulatory setting
Centre Hospitalier Universitaire Vaudois (CHUV)
Lausanne, Canton of Vaud, Switzerland
RECRUITINGCortisol levels in 4 biologicals fluids
Comparison of cortisol levels in these 4 biological fluids
Time frame: 24 hours
Aldosterone levels in 4 biological fluids
Comparison of aldosterone levels in these 4 biological fluids
Time frame: 24 hours
Glucocorticoid - comparison of repeated measures of glucocorticoid concentrations (cortisol, 18OHF, cortisone) profiles - over a period of 24 hours
Study of the diurnal rhythm and pulsatility in healthy participants (and later patients) * Diurnal rhythm: cosinor analysis. Comparison of nadir/acme of cortisol concentrations (and 18OHF, cortisone) among participants. Amplitude of the curve * Pulsatility analysis: frequency of the pulses and amplitude
Time frame: 24 hours
Mineralocorticoid - comparison of repeated measures of aldosterone concentrations profiles - over a period of 24 hours
Study of the diurnal rhythm and pulsatility in healthy participants (and later patients) * Diurnal rhythm: cosinor analysis. Comparison of nadir/acme of aldosterone concentrations among participants. Amplitude of the curve * Pulsatility analysis: frequency of the pulses and amplitude
Time frame: 24 hours
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