Randomized, double-blind, placebo-controlled crossover study designed to determine the effects of increasing colonic short-chain fatty acid (SCFA) content on aerobic endurance in healthy adults, and to identify underpinning mechanisms. In random order, healthy physically active adults will consume provided diets low in fiber and supplemented with SCFA-enriched high amylose maize starch (a poorly digested resistant starch considered a fermentable fiber) or low amylose maize starch (a rapidly digestible starch) for 1-week separated by a ≥2-week washout. At the end of each intervention period, participants will complete an endurance exercise bout followed by a time trial. Biological samples will be collected to assess muscle and whole body metabolism, gut microbiota, inflammation, and gastrointestinal function.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
QUADRUPLE
Enrollment
21
High-amylose maize starch (HAMS), commercially available as Hylon VII (Ingredion, Inc.), to which the SCFA acetate or butyrate has been chemically added. Hylon VII is a HAMS containing \~70% amylose and used in a variety of food products. To produce SCFA-enriched HAMS, Hylon VII is chemically modified through esterification with the SCFA acetate or butyrate.
Rapidly digestible low-amylose maize starch (0% amylose, 100% amylopectin) sold commercially as AMIOCA (Ingredion, Inc).
United States Army Research Institute of Environmental Medicine
Natick, Massachusetts, United States
Run time
Time to complete a 5km treadmill run
Time frame: Phases 1 and 2, day 8
Muscle glycogen content
Muscle glycogen content
Time frame: Phases 1 and 2, day 8
Plasma glucose turnover
Glucose production, utilization and metabolic clearance rate measured during endurance steady state exercise using 6,6-\[2H2\] glucose tracer
Time frame: Phases 1 and 2, day 8
Whole body substrate oxidation
Carbohydrate and fat oxidation rates measured during endurance steady state exercise using indirect calorimetry
Time frame: Phase 1 and 2, day 8
Serum short-chain fatty acid concentrations
Serum acetate, butyrate and propionate concentrations
Time frame: Phases 1 and 2, day 8. Measured fasted (-300 minutes) and before (0 minutes) and at the end (80 minutes) of steady state endurance exercise
Fecal short-chain fatty acid concentrations
Fecal acetate, butyrate and propionate concentrations
Time frame: Phases 1 and 2, days 1 and 7
Intestinal permeability
Small and large intestine permeability measured by saccharide excretion in urine
Time frame: Phases 1 and 2, day 8
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.