Pre-workout supplements (PWS) consumption in recreationally or physically trained males lead to many performance-enhancing benefits, including improvements in mean power output during single and repeated sprints, agility, reaction times, lower body muscular endurance and reduced fatigue. PWS ingestion also improves anaerobic performance and prolongs time to exhaustion during high-intensity intermittent exercise. However, PWSs' effectiveness is not constant, as they do not alter anaerobic power, jumping performance or blood lactate concentrations after a training session, at least in recreationally trained males and strength-power athletes. Moreover, the effects of long-term PWS supplementation, where some nutritional agents were combined (e.g., β-alanine, creatine, citrulline malate, etc.) to assess endurance-trained runners or elite cyclists' performance, are mixed and less clear. Even though the popularity of PWS use has increased among trained/professional athletes, most of the data in this area are derived from recreationally and not from well-trained athletes of a competitive level (especially in team sports). Therefore, the present study aimed to examine the acute and chronic effects of a PWS, containing 200 mg caffeine, 3.3 g creatine monohydrate, 3.2 g βalanine, 6 g citrulline malate and 5 g BCAA per dose, on shooting, jumping, sprinting, agility, aerobic and anaerobic performance in well-trained basketball players.
According to a randomized, double-blind, placebo-controlled, research design, 36 well-trained male basketball players will be recruited. During their first visit, they will complete a health history and exercise questionnaire and will sign a written informed consent. Participants' dietary profile will be evaluated using 3- day diet recalls and they will be instructed to follow the same dietary scheme for 3 days prior to the subsequent evaluations. During the second visit, participants will be familiarized with the performance evaluations (shooting, jumping, sprinting, agility, running-based anaerobic sprint test and Yo-Yo intermittent recovery level 1 test). (Acute phase) One week later, Moreover, they will randomly be assigned in two groups (PWS and placebo) by a research member not involved in data collection. After that participants will visit the basketball court in the morning and a blood sample will be collected after overnight fasting. Afterward, the participants in each group will perform the evaluations without PWS or PL. The evaluations will be performed according to National Strength and Conditioning Association standards. Body composition, resting heart rate, blood pressure, basketball-specific shooting exercise, counter-movement jump, 20-m sprint, agility T-Test, running-based anaerobic sprint test and Yo-Yo intermittent recovery level 1 test. One week later, all participants will visit the same basketball court at the same hour and will consume the PWS or placebo supplement and 30 minutes later their physiological characteristics and performance will be evaluated in the same sequence. (Chronic phase) Following the evaluations of the acute phase, participants will continue the consumption of the PWS or placebo, according to their allocation group, daily for a period of 4 weeks. At the end of the supplementation period, participants will visit the basketball court and a second blood sample will be collected after overnight fasting (the first blood sample, collected before the acute phase evaluations, will serve as baseline). Then, participants' performance will be evaluated in the same sequence as the previous visit.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
QUADRUPLE
Enrollment
36
Administration of a multi-ingredient dietary supplement consisting of 200 mg caffeine, 3.3 g creatine monohydrate, 3.2 g β-alanine, 6 g citrulline malate and 5 g branched chain amino acids
Administration of an isoenergetic placebo comparator dietary supplement consisting of 97% maltodextrin
Sports Nutrition Laboratory, International Hellenic University
Thessaloniki, Greece
Countermovement jump height
Jumping performance will be evaluated using a contact platform
Time frame: baseline and up to 4 weeks
20 meters sprint running time
Sprint performance will be evaluated using photocell gates
Time frame: baseline and up to 4 weeks
Rate of agility T-test performance
Agility T-test will be evaluated using photocell gates
Time frame: baseline and up to 4 weeks
Rate of running-based anaerobic sprint test performance
Running-based anaerobic sprint test performance will be evaluated using photocell gates
Time frame: baseline and up to 4 weeks
Rate of Yo-Yo intermittent recovery test level 1 test performance
Yo-Yo intermittent recovery test level 1 test performance will be evaluated using a field-based test
Time frame: baseline and up to 4 weeks
Rate of basketball-specific shooting performance
Basketball-specific shooting performance will be evaluated with a test consisting of 30 free shoots, 30 middle-distance shoots and 30 long-distance shoots
Time frame: baseline and up to 4 weeks
Blood lactate concentartion
Blood lactate concentration will be measured using a portable blood lactate analyzer
Time frame: baseline and up to 4 weeks
Creatine kinase concentration
Creatine kinase activity will be measured on a clinical chemistry analyzer
Time frame: baseline and up to 4 weeks
Lactate dehydrogenase concentration
Lactate dehydrogenase will be measured on a clinical chemistry analyzer
Time frame: baseline and up to 4 weeks
Alkaline phosphatase concentration
Alkaline phosphatase will be measured on a clinical chemistry analyzer
Time frame: baseline and up to 4 weeks
Gamma-glutamyltransferase concentration
Gamma-glutamyltransferase will be measured on a clinical chemistry analyzer
Time frame: baseline and up to 4 weeks
Urea concentration
Urea will be measured on a clinical chemistry analyzer
Time frame: baseline and up to 4 weeks
Creatinine concentration
Creatinine will be measured on a clinical chemistry analyzer
Time frame: baseline and up to 4 weeks
Serum glutamic-oxaloacetic transaminase activity
Serum glutamic-oxaloacetic transaminase will be measured on a clinical chemistry analyzer
Time frame: baseline and up to 4 weeks
Serum glutamate-pyruvate transaminase activity
Serum glutamate-pyruvate transaminase will be measured on a clinical chemistry analyzer
Time frame: baseline and up to 4 weeks
Body mass
Body mass will be measured using a calibrated digital scale
Time frame: baseline and up to 4 weeks
Body height
Body height will be measured using a stadiometer
Time frame: baseline and up to 4 weeks
Body fat mass
Body fat mass will be measured using a calibrated digital scale via bioelectrical impedance analysis
Time frame: baseline and up to 4 weeks
Systolic and diastolic blood pressure
Systolic and diastolic blood pressure will be measured using a blood pressure monitor
Time frame: baseline and up to 4 weeks
Heart rate
Heart rate will be measured using a heart rate monitor
Time frame: baseline and up to 4 weeks
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