The Mannheim Multicomponent Stress Test (MMST) is a validated laboratory stress test that combines cognitive, emotional, acoustic and motivational stress components. However the utility of the MMST as a viable alternative to the more commonly used Trier social stress test (TSST) to elicit HPA reactivity remains unclear as meaningful increases in saliva cortisol (\> 2.5 nmol/l) have been shown to occur in \<50% of participants yet the TSST typically elicits meaningful increases in saliva cortisol in \>70% of participants; likely as a consequence of the greater social evaluative component in the TSST. Using a randomised between groups design, this study aims to compare psychobiological responses to the MMST and TSST.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
31
Participants randomised to the MMST group will complete the computer based paced auditory serial addition task (PASAT-C) in the presence of one observer and is informed that incorrect answers result in reduced monetary compensation. Simultaneously, the participant is exposed to emotionally evocative images and white noise via headphones
Participants randomly assigned to the TSST condition will be exposed to a traditional TSST protocol. The TSST consists of a preparation phase (5 min), followed by a mock job interview and a mental arithmetic task (5 min each) in front of a panel consisting of two observers and a video recording device.
Faculty of Science, Liverpool John Moores University
Liverpool, Merseyside, United Kingdom
Change in saliva cortisol (pre-post stress)
Changes in the concentration of saliva free cortisol assessed by ELISA.
Time frame: Change from 5 minutes pre to peak post stress test (MMST or TSST)
State anxiety inventory (STAI-S) response to acute psychological stress
assessed using the state scale of the state trait anxiety inventory (STAI-S), consisting of a 20-item scale for measuring the intensity of anxiety as an emotional state (S-Anxiety). The range of possible scores for form Y of the STAI varies from a minimum score of 20 to a maximum score of 80. STAI scores are commonly classified as "no or low anxiety" (20-37), "moderate anxiety" (38-44), and "high anxiety" (45-80)
Time frame: Change from 5 minutes pre to after stress challenge (immediately after acute psychological stress and 60 minutes after the test) will be compared.
Heart rate
Continuous measurement of heart rate will be assessed using a telemetric chest strap.
Time frame: Continuously assessed from 30 minutes before to 60 minutes after the stress tests and control tests
Salivary alpha amylase (sAA)
a biomarker for stress-induced activity of the sympathetic nervous system (SNS), assessed by ELISA.
Time frame: Change from 5 minutes pre to immediately post stress test
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