Dileucine and Resistance Training Adaptations

Lindenwood University34 enrolled

Overview

This study seeks to compare the observed changes in resistance training adaptations after supplementation of isomolar amounts of leucine or dileucine in healthy resistance-trained men. This will provide a better understanding of supplementation with leucine versus dileucine over a 10-week period in regards to increasing muscular performance.

The study will be conducted using a randomized, double-blind approach with individuals being provided daily 2-gram doses of leucine, 2-gram doses of dileucine, or a placebo. Daily blinded supplementation will occur each day for a 10-week period of time while completing a heavy resistance training program. Participants follow the resistance training program for a total of 10 weeks. To compare the efficacy of leucine in form of amino acid or dipeptide supplementation on resistance training adaptations, changes in fat-free, lean, and fat mass will be determined using a 4-compartment body composition model. Skeletal muscle cross-sectional area will be assessed using ultrasound. Maximal strength, muscular endurance, and power will also be assessed. Participants will be required to provide weekly compliance and complete one supervised workout per week. After 2, 6, and 10 weeks of following the supplementation and resistance training regimens, participants will return to the laboratory to complete testing bouts consisting of identical assessments of body composition, muscular strength, muscular endurance, lower-body power, and anaerobic capacity. Adverse events related to each supplementation group will be recorded and assessed.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

OTHER

Masking

DOUBLE

Enrollment

Conditions

Hypertrophy Strength Training Adaptations Weight-Bearing Strengthening Program

Interventions

PlaceboDIETARY_SUPPLEMENT

Participants will supplement daily for 10 weeks with 2 grams rice flower placebo in capsule form. On training days, participants will consume their assigned supplement within 60 minutes of completing their workout. On non-training days, participants will consume their assigned supplement with their first meal of the day. All doses will be consumed with 8-12 ounces of water.

LeucineDIETARY_SUPPLEMENT

Participants will supplement daily for 10 weeks with 2 grams leucine in capsule form. On training days, participants will consume their assigned supplement within 60 minutes of completing their workout. On non-training days, participants will consume their assigned supplement with their first meal of the day. All doses will be consumed with 8-12 ounces of water.

DileucineDIETARY_SUPPLEMENT

Eligibility

Sex: MALEMin age: 18 YearsMax age: 39 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Male * Between the ages of 18-39 years of age * Currently participating in resistance training exercise Exclusion Criteria: * Female * Participants who are determined to not be weight stable defined as week 0 and week 2 body mass levels deviating by 3% or more. * Any individual who is currently being treated for or diagnosed with a cardiac, respiratory, circulatory, musculoskeletal, metabolic, immune, autoimmune, psychiatric, hematological, neurological or endocrinological disorder or disease. * Body mass index \> 25 kg/m2. Individuals with a body mass index greater than 25 kg/m2, but a body fat percentage less than 25% fat will be accepted into the study * Individuals who present with any previous injury or illness that would prevent them from appropriately completing all exercise lifts. * Participants who are not able to fit onto the DEXA table will not be able to participate in the research due to size restrictions of the equipment. This typically includes individuals exceeding 400 pounds or those greater than 6'4". * Those individuals with less than 12 months of structured resistance training experience will be excluded from the study. * Individuals whose maximal relative upper body strength is below 1.0 will be excluded. * Individuals whose maximal relative lower body strength is below 1.5 will be excluded. * All participants who DO NOT abstain from taking any additional forms of nutritional supplementation deemed to be ergogenic or that will impact resistance training adaptations (pre-workouts, creatine, beta-alanine, etc.) for four weeks prior to beginning this study and for the entire duration of the study. * Individuals who are currently using or have used within the past 12 months anabolic-androgenic steroids.

Locations (1)

Exercise and Performance Nutrition Laboratory

Saint Charles, Missouri, United States

Outcomes

Primary Outcomes

Fat-Free Mass

Fat-free mass will be determined via Dual-Energy X-Ray Absorptiometry

Time frame: 10 weeks

Leg Press 1RM

Leg Press 1RM will be measured using the leg press (lower-body) exercise.

Time frame: 10 weeks

Bench Press 1RM

Bench Press 1RM will be measured using the bench press (upper-body) exercise.

Time frame: 10 weeks

Maximal Isometric Mid-Thigh Pull Force Production

Maximal Isometric Mid-Thigh Pull Force Production will be measured using an isometric mid-thigh pull test

Time frame: 10 weeks

Secondary Outcomes

Fat Mass

Fat mass will be determined via Dual-Energy X-Ray Absorptiometry

Time frame: 10 weeks

Lean mass

Lean mass will be determined via Dual-Energy X-Ray Absorptiometry

Time frame: 10 weeks

% Body Fat

% Body Fat will be determined via Dual-Energy X-Ray Absorptiometry

Time frame: 10 weeks

Total body water

Total Body Water will be determined via bioelectrical impedance analysis

Time frame: 10 weeks

Extracellular Body Water

Extracellular Body Water will be determined via bioelectrical impedance analysis

Data from ClinicalTrials.gov

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