This study focuses on the role of neutrophils in shaping the adaptive immune response to the anti-pneumococcal vaccine Prevnar-13 in young and elderly adults.
This study focuses on the role of neutrophils in shaping the adaptive immune response to the anti-pneumococcal vaccine Prevnar in young and elderly adults. This study involves direct vaccination of human volunteers. The endpoints will be determining how vaccination affects neutrophil responses and linking those neutrophil responses to antibody responses following vaccination. The main results will be: 1. Elucidating how neutrophils kill bacteria before and after vaccination 2. Elucidate the phenotype of neutrophils before and after vaccination 3. Elucidate how neutrophils interact with B and T cell in vitro before and after vaccination 4. Measure antibody levels and function before and after vaccination 5. Correlate neutrophil responses to antibody levels and function
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
60
Vaccination against 20 serotypes of S. pneumoniae
University at Buffalo
Buffalo, New York, United States
RECRUITINGPneumococcal Opsonophagocytic Killing Activity (OPH) by neutrophils from young versus old donors at 1 week and 1 month after vaccination
OPH will be determined by measuring the percentage of S. pneumoniae bacteria killed in vitro by neutrophils from donors
Time frame: 1 month
Surface phenotype of neutrophils from young versus old donors at 1 week and 1 month after vaccination
Phenotype will be determined by measuring the expression of surface receptors on neutrophils ex vivo by flowcytometry from donors
Time frame: 1 month
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