Echointensity and echotexture have been used as a physiological marker for changes in skeletal muscle quality and structure caused by physical training, low activity, ageing and some neuromuscular disorders. However, there are some influencing factors on muscle echo-intensity and echotexture, such as temperature, which may not be taken into account when performing an ultrasound assessment and may alter the results. This study aims to investigate the effects of muscle temperature on echointensity and other 2nd order echotextures variables such as homogeneity, contrast, correlation and entropy of muscle tissue, in order to gain a better understanding of this correlation and minimise its influence, which would allow greater precision in the use of muscle ultrasound as a diagnostic tool. The methodology of this study includes firstly the acquisition of ultrasound images of the vastus lateralis muscle. This acquisition will be performed during the continuous recording of muscle temperature, carried out during a passive cooling process after 20 min of heating using microwave equipment. In addition, to standardise the results, a correction factor will be calculated to compensate for the influence of subcutaneous adipose tissue thickness on echogenicity and echotexture.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
OTHER
Masking
NONE
Enrollment
30
Passive heating of the vastus lateralis of the right quadriceps will be performed using a microwave device. The device will be set at a power of 150 W and a distance of 10-15 cm from the skin surface for 20 minutes with the purpose of increasing local muscle temperature to 40 ºC.
Sergio Montero Navarro
Elche, Alicante, Spain
RECRUITINGMuscle echointensity (0-255 a.u.).
Muscle echointensity is calculated by the mean gray level value of the muscle Range of Interest (ROI). (0-255 a.u.)
Time frame: During intervention
Muscle echovariance (0-255 a.u.).
The echovariance is determined by the relationship between the standard deviation and the average pixel intensity obtained from the histogram of the muscle ROI. (0-255 a.u.)
Time frame: During intervention
Muscle energy or second angular momentum.
It is a second order parameter (GLCM) that measures textural uniformity of the muscle ROI
Time frame: During intervention
Muscle homogeneity.
It is a second-order parameter (GLCM) that measures the uniformity of the muscle ROI composition.
Time frame: During intervention
Contrast.
It is a second-order parameter (GLCM) that measures local variations in gray levels of the muscle ROI.
Time frame: During intervention
Textural Correlation.
It is a second-order parameter (GLCM) that expresses linear gray-level dependencies of the muscle ROI.
Time frame: During intervention
Entropy.
It is a second-order parameter (GLCM) to measure muscle ROI disorder.
Time frame: During intervention
Echointensity correction factor for adipose thickness
The measurement of subcutaneous adipose thickness will be carried out through the average of the distance from the skin to the aponeurosis coinciding with the superficial interface of the muscle and with different pressures of the probe on the skin. This measure will be used to develop a correction factor that allows compensating the influence of this variable on echogenicity and echotexture, standardizing the results.
Time frame: Pre-intervention
Muscle temperature (ºC).
Measurement of intramuscular temperature of the right vastus lateralis of the quadriceps (ºC)
Time frame: Pre-intervention and during intervention
Age (years)
Subject's age (years)
Time frame: Pre-intervention
Sex (female or male)
Subject's sex (female or male)
Time frame: Pre-intervention
Dominance
Dominant leg (right-left)
Time frame: Pre-intervention
Body mass index
It is a measure of body fat based on height and weight that applies to adult men and women
Time frame: Pre-intervention
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