Effects of Pea Proteins With Different Degrees of Processing on Postprandial Metabolism in Healthy Adults

University of Bonn20 enrolled

Overview

The aim of this study is to investigate the postprandial metabolic responses to plant-based protein ingredients of different degrees of processing (pea protein concentrate, isolate, and extrudate) and in comparison to whey protein in healthy adults. Therefore, young healthy subjects consume 4 test meals with 25 g of protein from pea protein concentrate, pea protein isolate, pea protein extrudate or whey protein in a randomized order. In a postprandial period of 6 hours, parameters of protein and glucose metabolism (i.a. plasma amino acids), gastric emptying and hunger/satiety are analysed. It is assumed that the plasma amino acid profile after plant protein ingestion differs depending on processing level of the protein ingredients and in comparison to whey protein.

In a randomized crossover-design, 20 healthy women and men consume 4 test meals containing either 25 g of protein from plant protein ingredients (pea protein concentrate, isolate, or extrudate) or whey protein together with 1 g paracetamol, which is used as a marker for gastric emptying. During a postprandial period of 6 hours parameters of protein metabolism (e.g. amino acids in plasma and urine), glucose metabolism (plasma glucose, serum insulin) and gastric emptying rate (based on plasma paracetamol) are analysed. Furthermore, subjective hunger/satiety and acceptance of the meals are assed using visual analogue scales. Each intervention arm will be separated by a washout period of at least 7 days.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Plasma Amino Acid Appearance and Disappearance

Eligibility

Sex: ALLMin age: 20 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * non-smoking * metabolically healthy * normal weight (BMI: 18,5 - 24,9 kg/m2) * written informed consent Exclusion Criteria: * pregnancy, lactation * hypo- or hypertension * underweight or overweight/obesity * food intolerances and allergies (especially milk protein, pea, celery) * malabsorption syndromes * gastrointestinal diseases * thyroid diseases * diabetes mellitus type 1 and type 2 * impaired kidney or liver function * anaemia * blood coagulation disorders * irregular menstrual cycle * endometriosis, severe menstrual problems * hormonal contraception * regular use of medications (especially habitual use of paracetamol or medications that may interact with paracetamol) * hypersensitivity to paracetamol * body weight below 50 kg * alcohol abuse * glucose-6-phosphate dehydrogenase deficiency * Gilbert's syndrome * vegan diet * eating disorders (especially anorexia nervosa, bulimia nervosa) * smoking * participation in another study

Outcomes

Primary Outcomes

Amino acid profile in blood

Analysis of amino acid profile in plasma (nmol/ml)

Time frame: Postprandial period of 5 hours (Time points: fasting (0) and 15, 30, 45, 60, 90, 120, 180, 240 and 300 minutes)