Clinical Trial of 2-HOBA in Pulmonary Arterial Hypertension

Overview

Based on existing literature and clinical trials, 2- hydroxbenzylamine (2-HOBA) has clear impact on mechanisms that much of the international field of pulmonary hypertension (PH) research agrees are central to disease progression. The investigator's preliminary data and Phase I studies demonstrate not only a clear positive impact on reducing pulmonary vascular resistances in Group I and II PH, and both cytokine and molecular biomarkers of disease, but also indicated the potential for a substantial positive effect on heart function under load stress. In this Phase II project, investigators will test the safety and efficacy of 2-HOBA in PH patients, improving the function of the right ventricle under stress in a large animal model, and effectiveness in the context of standard-of-care in mouse models and large animals, to establish the remaining data needed to proceed to commercialization.

Pulmonary Hypertension (PH) is a disease in which gradual cellular occlusion of the blood vessels in the lungs increases pulmonary vascular resistance, eventually leading to right heart failure and death. Approved therapies in common use are all different classes of vasodilator, which can extend life in some patients, but are not disease modifying and do not prevent decline and death. There is thus need for new therapies for PH. Although originating causes are different across patients, a common molecular factor across most etiologies is an increase in reactive oxygen species leading to a set of specific metabolic problems. These include increased insulin resistance, increased reliance on glutamine uptake in the lungs, a shift away from normal TCA-glucose metabolism, and suppressed fatty acid oxidation in the heart leading to increased intracellular fat. An important part of the mechanism is a feedback loop in which ROS causes reactive lipids particularly within the mitochondria, which then adduct to proteins causing loss of function and further metabolic defects. One such target in PH is the mitochondrial lysine deacetylase, Sirt3. In a recent clinical trial attempting an intervention on this metabolic axis, the only patients in whom it succeeded were those with an unusual activating set of SNPs in Sirt3, indicating that rescue of Sirt3 is a prerequisite for resolving disease. This presents the possibility of rescuing the defects in metabolic function that are required for disease progression by alleviating the upstream problem with reactive lipid species. 2-Hydroxybenzylamine (2-HOBA) is a potent scavenger of reactive lipid dicarbonyls. It blocks the effects of reactive lipids by selectively trapping them - giving an alternate target to bind to with a binding affinity two to three orders of magnitude stronger. We have published that 2-HOBA treatment rescued Sirt3 activity in vivo in BMPR2 mutant mice, resulting in normalization of metabolic markers and near normalization of pulmonary vascular resistance2, resulting in reduced acetylation of key targets. In Phase I of this project, we found that 2-HOBA also reduced pulmonary pressures in the AKR-high fat diet model of Group II PH, the most common form of disease, associated with metabolic syndrome. The investigators also identified cytokines and metabolites known to be changed in PH that were corrected in our models, which could be used as biomarkers. Metabolic changes in the hearts in response to 2-HOBA in both mouse models which the investigators believe are extremely positive - increased ability to metabolize fatty acids, which is a key need in the right ventricle under stress. The investigator's preliminary studies show that 2-HOBA is a safe compound which shows great promise in treating the core molecular defect in PH, in both the closest genetic mouse model to human disease available (BMPR2 mutants), in a mouse model of the most common type of PH (AKR-high fat diet) and in existing and animal toxicity and human safety trials. Based on existing literature and clinical trials, 2- hydroxbenzylamine (2-HOBA) has clear impact on mechanisms that much of the international field of pulmonary hypertension (PH) research agrees are central to disease progression. Our preliminary data and Phase I studies demonstrate not only clear positive impact on reducing pulmonary vascular resistances in Group I and II PH, and both cytokine and molecular biomarkers of disease, but also indicated the potential for a substantial positive effect on heart function under load stress. In this Phase II project, investigators will test the safety and efficacy of 2-HOBA in PH patients, improving function of the right ventricle under stress in a large animal model, and effectiveness in the context of standard-of-care in mouse models and large animals, to establish the remaining data needed to proceed to commercialization.