The objective of this study is to determine the effect of consumption of drinks with varying amounts of glycerol and electrolytes on fluid balance at rest.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
QUADRUPLE
Enrollment
37
9.3 oz (273 ml) aliquot of one of the study beverages every 15 min for a total of 28 oz (828 ml) over a 45-min period, followed by 15 min of rest. Flavored water matched for sweetness, color. flavor, but cannot be matched for saltiness. All sodium-containing drinks will include a base blend of other electrolytes (potassium 15 mmol/L, magnesium 8 mmol/L, and calcium 5 mmol/L).
PepsiCo R&D, Gatorade Sports Science Institute
Valhalla, New York, United States
Fluid balance
Change in body mass to the nearest 0.005 kilogram (kg) using a platform scale
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 30, 60, 90,120 and 180 minutes
Fluid retention
As a percent (%)
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 30, 60, 90,120 and 180 minutes
Urine mass
By urine mass scale in grams (g)
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 30, 60, 90, 120 and 180 minutes
Urine specific gravity
By USG digital pen refractometer
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 30, 60, 90,120 and 180 minutes
Blood sodium
By iSTAT Blood Electrolyte and Chemistry Analyzer (CG8+ cartridge) in mmol/L
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 60 min, 120 min and 180 minutes
Blood glucose
Glucometer for point of care test in mg/dL
Time frame: Screening visit only
Blood glucose
By iSTAT Blood Electrolyte and Chemistry Analyzer (CG8+ cartridge) in mg/dL
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 60 min, 120 min and 180 minutes
Percent change in plasma volume
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Calculated from hematocrit and hemoglobin concentration
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 60 min, 120 min and 180 minutes
Serum glycerol concentration
By Sigma Aldrich glycerol assay kit in mmol/L
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 60 min, 120 min and 180 minutes
Blood pressure
mmHg by automatic sphygmomanometer
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 30, 60, 90, 120 and 180 minutes
Heart rate
Beats per minute (bpm) by heart rate monitor
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 30, 60, 90, 120 and 180 minutes
Subjective measures of gastrointestinal discomfort and headache
Visual Analog Scale (VAS) symptom rating of how you feel right now by placing a slash along a 100 millimeter (mm) vertical line anchored from 0 mm (None) to 100 mm (Severe). Fewer symptoms and lower numbers are better.
Time frame: Change from baseline (pre-beverage) to post-beverage Time 0, 30, 60, 90, 120 and 180 minutes
Menstrual cycle phase
Date of last menstrual period by self-report of female participant
Time frame: Female participants log menstrual flow throughout study participation. The three study sessions (2-45 days apart) are scheduled to avoid menses and can be completed in the same menstrual cycle, or in 3 different cycles (3 different months) if needed.