The goal of this trial is to examine the effects of a single high-fat meal with or without the co-ingestion of dietary nitrate on exercise-mediated sympatholysis. The main questions it aims to answer are: 1. What are the mechanisms responsible for reduced exercise-mediated sympatholysis following a high-fat meal 2. Can dietary nitrate prevent the declines in exercise-mediated sympatholysis Participants will be asked to complete three study visits in a randomized order: 1. Low-fat meal 2. High-fat meal 3. High-fat meal plus dietary nitrate Exercise-mediated sympatholysis will be measured using Doppler ultrasound via a protocol involving rhythmic handgrip exercise and a lower body negative pressure stimulus. Sympatholysis will be assessed before each meal, and at 1, 2, and 3 hours post-prandial.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
15
Drink containing 10g of beetroot powder in 250 mL cold tap water
Mio flavouring in 250 mL cold tap water. Trace amounts of sugar and sodium were added to match the content in the beetroot supplement
Low-fat meal with cornflakes, greek yogurt, skim milk, and orange juice
University of Guelph - Human Cardiovascular Physiology Laboratory
Guelph, Ontario, Canada
Forearm vascular conductance
Duplex ultrasound of brachial artery
Time frame: Before intervention and 1, 2, 3 hours post-intervention
Forearm blood flow
Duplex ultrasound of brachial artery
Time frame: Before intervention and 1, 2, 3 hours post-intervention
Systolic and Diastolic Blood Pressure
finger plethysmography
Time frame: Before intervention and 1, 2, 3 hours post-intervention
Plasma levels of Norepinephrine
ELISA kit
Time frame: Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date
Plasma levels of Nitrate/Nitrite concentration
ELISA kit
Time frame: Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date
Blood Glucose concentrations
Blood glucose monitor
Time frame: Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood analyzed immediately after collection at each time point
Plasma levels of Triglycerides
Triglyceride assay kit
Time frame: Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date
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