The aim of this study is; detection of Galectin-10, Galectin-7, MMP-9 levels in saliva samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples of saliva were obtained from 60 systemically healthy non-smoker individuals with periodontitis (P, n=20), gingivitis(G, n=20) and healthy periodontium (S, n=20). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine Galectin-10, Galectin-7 and MMP-9 levels in the biological samples.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
60
The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis.
Izmir Katip Çelebi University Department of Periodontology
Izmir, Çiğli, Turkey (Türkiye)
RECRUITINGThe total amount of Gal-10 in saliva
The total amount of Galectin-10 in saliva
Time frame: 24 hours after taking the clinical measurements at the first visit
The total amount of Gal-7 in saliva
The total amount of Galectin-7 in saliva
Time frame: 24 hours after taking the clinical measurements at the first visit
The total amount of MMP-9 in saliva
The total amount of Matrix metalloproteinase 9 in saliva
Time frame: 24 hours after taking the clinical measurements at the first visit
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