The purpose of this project is to investigate underlying mechanisms of testosterone- and resistance training-induced muscle hypertrophy and muscle memory in humans.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
QUADRUPLE
Enrollment
30
Following testosterone/placebo injection, participants undergo supervised resistance training for 4 weeks. This period is followed by a washout period with no drug and either continued resistance training or detraining for 16 weeks. Thereafter, another 4-week period with supervised resistance training is commenced, with no drug.
The August Krogh Section for Human and Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen
Copenhagen, Denmark
RECRUITINGFiber cross-sectional area
Immunohistochemistry to determine fiber cross-sectional area
Time frame: The change in fiber cross-sectional is assessed at baseline, 4, 20 and 24 weeks
Myonuclei
Immunohistochemistry to determine number of myonuclei
Time frame: The change in myonuclei is assessed at baseline, 4, 20 and 24 weeks
Skeletal muscle proteome
Pathway enrichment analysis of the muscle proteome using mass spectrometry-based proteomics
Time frame: The change in the muscle proteome is assessed at baseline, 4, 20 and 24 weeks
Lean mass
Lean mass assessed by dual X-ray absorptiometry (DXA)
Time frame: The change in lean mass is assessed at baseline, 4, 20 and 24 weeks
Satellite cells
Immunohistochemistry to determine number of satellite cells
Time frame: The change in satellite cells is assessed at baseline, 4, 20 and 24 weeks
Muscle strength
1-repetition maximum
Time frame: The change in 1-repetition maximum is assessed at baseline, 4, 20 and 24 weeks
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