Celiac disease (CD) is an autoimmune enteropathy triggered by the intake of gluten, characterized by a genetic predisposition. Although, CD is often associated with malabsorption symptoms, a growing number of affected subjects are overweight or frankly obese. One of the conditions that is most frequently detected in pauci/asymptomatic subjects is an increase in transaminases, which often regresses completely after the start of GFD. More recently, a specific liver disorder has shown a certain relevance in adult patients suffering from CD, so much so that the European Society for the Study of Coeliac Disease (ESsCD) has cited it among the possible comorbidities which should be screened in CD subjects: Non-Alcoholic Fatty Liver Disease (NAFLD). In adults, a non-random association between CD and NAFLD has been demonstrated, showing a CD prevalence rate of 2-14% among patients with NAFLD. Few studies have focused on this same aspect in pediatric age, reporting contrasting data. Several factors have been advocated as putative responsible of association between CD and NAFLD: dietary imbalances, intestinal mucosa permeability impairment, alterations of the intestinal microbiota. The objectives of this study are: 1. define, retrospectively, the prevalence of NAFLD in a pediatric population affected by CD and study its possible association with GFD. 2. define the possible role of the intestinal permeability alteration and/or the intestinal mucosa damage and/or the proinflammatory status in the development of NAFLD in children affected by CD.
Background Celiac disease (CD), an autoimmune enteropathy triggered by the intake of gluten, is a widespread pathology, with a worldwide estimated prevalence of approximately 1%, characterized by both gastrointestinal and systemic symptoms, both in adults and children, which are usually resolved by eliminating gluten from the diet (Gluten-Free Diet, GFD). However, it still remains an under-diagnosed condition (to date the overall prevalence in Italy is 0.37% and in Sicily 0.35%). Although, CD is often associated with malabsorption symptoms (steatorrhea, weight loss, nutritional deficits, etc.), a growing number of affected subjects are overweight or frankly obese. One of the conditions that is most frequently detected in pauci/asymptomatic subjects is an increase in transaminases, which often regresses completely after the start of GFD. More recently, a specific liver disorder has shown a certain relevance in patients suffering from CD, so much so that the European Society for the Study of Coeliac Disease (ESsCD) has cited it among the possible comorbidities which should be screened in CD subjects: Non-Alcoholic Fatty Liver Disease (NAFLD). This condition affects approximately 34% of overweight or frankly obese children and is closely related, both in children and adults, to the traits of metabolic syndrome, so much so that today it is preferably referred to as Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD). In adults, a non-random association between CD and NAFLD has been demonstrated, showing a CD prevalence of 2-14% among patients with NAFLD. Few studies have focused on this same aspect in pediatric age, however reporting that the prevalence of CD among children with NAFLD could be comparable to the prevalence of CD in the general pediatric population. Nevertheless, a study conducted on 11488 children (0-19 years) affected by CD compared with 57029 healthy children supports the hypothesis of a close association between CD and NAFLD in childhood. Authors proved a 4.6 relative risk value of NAFLD in CD patients. Several factors have been advocated as putative responsible for this association: 1) dietary imbalances due to the adoption of GFD, rich in fats and sugars and low in fiber; 2) alteration of intestinal permeability, with an increase in the translocation of bacterial substances from the gut, which, are transported directly to the liver, where they are able to generate inflammatory processes mediated by the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB), activated by the Tool-like receptor 4 (TLR4)-Lipopolysaccharide (LPS)-LPS Binding Protein (LPB) complex, with production of proinflammatory cytokines \[Tumor Necrosis Factor (TNF)-α, Interleukine (IL)-1β, IL-6, IL-12, IL-18); 3) alterations of the intestinal microbiota. Objectives Primary objective: define, retrospectively, the prevalence of NAFLD in a pediatric population affected by CD and study its possible association with GFD: Objective 1.1: identify the prevalence of NAFLD before the introduction of the GFD in an already diagnosed pediatric celiac; Objective 1.2: identify the prevalence of NAFLD after at least 12 months of GFD in an already diagnosed pediatric celiac; Objective 1.3: compare the prevalence of NAFLD before and after (at least 12 months) the introduction of the GFD in all pediatric celiac patients for whom both pre- and post-GFD data are available; Objective 1.4: establish a possible correlation between the presence/absence of NAFLD, both before and after the adoption of the GFD, and clinical, laboratory, immunological, genetic and histological parameters, in order to identify a subgroup of celiac children potentially predisposed to the NAFLD development; Objective 1.5: establish any changes in the clinical, laboratory, immunological, genetic and histological parameters of the population referred to in objective 1.3 and establish whether there is a correlation between these and the possible onset/regression of NAFLD. Secondary objective: define the possible role of the intestinal permeability alteration and/or the intestinal mucosa damage and/or the proinflammatory status in the development of NAFLD in children affected by CD: Objective 2.1: identify the possible presence of serological markers of altered intestinal permeability and/or intestinal mucosa damage in a population of celiac children prospectively enrolled at the Pediatric Clinic of the Children's Hospital "G. Di Cristina" before the start of the GFD (T0). Objective 2.2: Identify the possible presence of blood markers of inflammation in the population referred to in point 2.1 at T0. Objective 2.3: define the existence of an association between the markers of altered intestinal permeability and/or mucosal damage and/or inflammation and the presence of NAFLD in the population referred to in point 2.1 at T0. Objective 2.4: evaluate any changes in serological markers of altered intestinal permeability and/or mucosal damage in the population referred to in point 2.1 after 6 months of strict adherence to the GFD (T1). Objective 2.5: evaluate any changes in blood markers of inflammation in the population referred to in point 2.1 at T1. Objective 2.6: define the existence of an association between the markers of altered intestinal permeability and/or mucosal damage and/or inflammation and the presence of NAFLD in the population referred to in point 2.1 at T1. Materials and methods Primary objective To achieve the primary objective of the study, all the medical records of pediatric patients suffering from CD already diagnosed according to the European Society for Paediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) criteria at the Pediatric Clinic of the Children's Hospital "G. Di Cristina" will be retrospectively analyzed. Objectives 1.1, 1.2 and 1.3 To achieve objectives 1.1 and 1.2, the presence and degree of steatosis diagnosed by abdominal ultrasound will be assessed before the start of the GFD (objective 1.1) or after at least 12 months from the introduction of the GFD (objective 1.2). To achieve objective 1.3, a sub-analysis of the presence and degree of steatosis will be performed in all those patients who have undergone an abdominal ultrasound both before and after the introduction of GFD. Objectives 1.4 and 1.5 To achieve objective 1.4, clinical (sex, ethnicity, age at diagnosis, BMI, symptoms, duration of GFD), laboratory (transaminasemia, albuminemia, total cholesterolemia and subclasses, triglyceridemia, fasting glycemia, insulinemia, Homeostasis Model Assessment-Insulin Resistance HOMA-IR Index), immunological \[anti-gliadin antibodies (AGA), anti-tissue transglutaminase antibodies (tTG), anti-endomysial antibodies (EMA)\], genetic (HLA structure) and histological (Marsh duodenal histological classification, presence of eosinophilic infiltration, etc.) data of all included patients will be collect, both before (target population 1.1) and after (target population 1.2) the introduction of the GFD. To achieve objective 1.5, the same clinical, laboratory, immunological, genetic and histological parameters analyzed for objective 1.4 will be analyzed, if available both before and after the introduction of the GFD, in order to establish whether there is a correlation between these and the possible onset/regression of NAFLD in the population referred to in objective 1.3. Secondary objective To achieve the secondary objective of the study, pediatric patients affected by newly diagnosed CD according to the ESPGHAN criteria will be prospectively enrolled at the at the Pediatric Clinic of the Children's Hospital "G. Di Cristina". The number of subjects to be enrolled will be equal to 91, considering, in the Province of Palermo, Sicily, Italy, a pediatric population (between 0 and 14 years) of 170,468 subjects, a prevalence of CD equal to 1%, a confidence level of 95% and a confidence interval of 10%. Furthermore, as the exact prevalence of NAFLD in children with CD it's not clearly defined, results derived from the retrospective part of this study (objective 1.1) will be used to maintain a proportion between subjects enrolled without and with pre-GFD NAFLD ("competitive enrollment"). Objectives 2.1, 2.2, 2.3 To achieve objective 2.1, all enrolled subjects, for whom the same clinical, laboratory, immunological, genetic and histological parameters analyzed for objective 1.4 must be available (the latter only if necessary for diagnosis), in order to make a comparison possible, will be subjected to blood sampling for the identification of serological markers of altered intestinal permeability \[zonulin (Zo), occludin (OCLN), anti-OCLN antibodies, claudin 1 (CLDN1) and anti-CLDN1 antibodies\] and mucosal damage \[Intestinal Fatty Acid Binding Protein 2 (iFABP2)\], lipopolysaccharide (LPS), lipopolysaccharide binding protein (LPB)\] before starting the GFD (T0). The blood sampling will coincide with the one necessary for the diagnostic definition of the patient's clinical picture. To achieve objective 2.2, all enrolled subjects will undergo a blood sample before starting the GFD (T0), for the identification of the following inflammatory markers: TNF-α, IL-1β, IL-6, IL-12, IL-18. The blood sampling will coincide with the one necessary for the diagnostic definition of the patient's clinical picture. To achieve objective 2.3, all enrolled subjects, before starting the GFD (T0), will undergo an abdominal ultrasound to study hepatic steatosis according to the methods previously described and a statistical correlation analysis will be carried out between the presence/absence of NAFLD and the markers assayed for objective 2.1 and 2.2. The assay of intestinal permeability and mucosal damage markers will be carried out using the ELISA method, with commercially available kits, following the manufacturers' instructions. The measurement of inflammation markers will be performed by intracellular staining with flow cytometric method on peripheral blood mononuclear cells (PBMC) extracted from patients' blood samples. Objectives 2.4, 2.5, 2.6 To achieve objective 2.4, all enrolled subjects, for whom the same clinical, laboratory and immunological parameters analyzed at T0 must be available, in order to make a comparison possible, will be subjected to a blood sample for the identification of serological markers of altered intestinal permeability and mucosal damage already measured at T0 (see objective 2.1), after 6 months of strict adherence to the GFD (T1). The blood sampling will coincide with the one necessary to re-evaluate the patient's clinical picture after 6 months of adherence to the GFD. To achieve objective 2.5, all enrolled subjects will undergo a blood sample to identify the inflammation markers already dosed at T0 (see objective 2.2), after 6 months of strict adherence to the GFD (T1). The blood sampling will coincide with the one necessary to re-evaluate the patient's clinical picture after 6 months of adherence to the GFD. To achieve objective 2.6, all enrolled subjects, after 6 months of strict adherence to the GFD (T1), will undergo abdominal ultrasound to study hepatic steatosis according to the methods previously described and a statistical correlation analysis will be carried out between the presence/absence of NAFLD and the markers assayed for objective 2.4 and 2.5. The assessment of adherence to the GFD will be carried out through the monthly compilation, by the children and/or their parents, of a specifically created and validated questionnaire.
Study Type
OBSERVATIONAL
Enrollment
91
Pediatric patients affected by newly diagnosed Celiac Disease (CD) according to the European Society for Paediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) criteria will start a gluten-free diet (GFD), as normally expected for all CD patients. The assessment of adherence to the GFD will be carried out through the monthly compilation, by the children and/or their parents, of a specifically created and validated questionnaire. Before and after the start of GFD enrolled patients will undergo an abdominal ultrasound to establish the presence and degree of hepatic steatosis.
Gastroenterological Paediatrics, Children's Hospital "G. Di Cristina"
Palermo, Palermo, Italy
RECRUITINGGeneral Pediatrics Unit, Children's Hospital "G. Di Cristina"
Palermo, Palermo, Italy
RECRUITINGHistorical Nonalcoholic Fatty Liver Disease Prevalence in Pediatric Celiac Disease Patients before Gluten Free Diet
We will review all the clinical charts of historical Celiac Disease pediatric patients to identify the number of patients suffering from Nonalcoholic Fatty Liver Disease before the start of gluten free diet. Hepatic steatosis will be ascertained by ultrasound examination and classified as follows: absent (score 0), present (score 1)
Time frame: Retrospective (years 2000-2023)
Historical Nonalcoholic Fatty Liver Disease Prevalence in Pediatric Celiac Disease Patients after Gluten Free Diet
We will review all the clinical charts of historical Celiac Disease pediatric patients to identify the number of patients suffering from Nonalcoholic Fatty Liver Disease after the start of gluten free diet (at least 1 year of diet). Hepatic steatosis will be ascertained by ultrasound examination and classified as follows: absent (score 0), present (score 1)
Time frame: Retrospective (years 2000-2023)
Historical Nonalcoholic Fatty Liver Disease Degree in Pediatric Celiac Disease Patients before Gluten Free Diet
We will review all the clinical charts of historical Celiac Disease pediatric patients to identify the steatosis degree of patients suffering from Nonalcoholic Fatty Liver Disease before the start of gluten free diet. Hepatic steatosis will be ascertained by ultrasound examination and classified as follows: when the echostructure of the liver is normal; mild (score 1 = mild), when there is a mild and diffuse increase in hepatic echogenicity, with normal visualization of the portal vein wall and diaphragm; moderate (score 2 = moderate), in case of moderate increase in hepatic echogenicity, with slightly altered appearance of the portal vein wall and diaphragm; severe (score 3 = severe), in case of marked increase in hepatic echogenicity, with poor or absent visualization of the wall of the portal vein, the diaphragm and the posterior part of the right hepatic lobe
Time frame: Retrospective (years 2000-2023)
Historical Nonalcoholic Fatty Liver Disease Degree in Pediatric Celiac Disease Patients after Gluten Free Diet
We will review all the clinical charts of historical Celiac Disease pediatric patients to identify the steatosis degree of patients suffering from Nonalcoholic Fatty Liver Disease after the start of gluten free diet (at least 1 year of diet). Hepatic steatosis will be ascertained by ultrasound examination and classified as follows: when the echostructure of the liver is normal; mild (score 1 = mild), when there is a mild and diffuse increase in hepatic echogenicity, with normal visualization of the portal vein wall and diaphragm; moderate (score 2 = moderate), in case of moderate increase in hepatic echogenicity, with slightly altered appearance of the portal vein wall and diaphragm; severe (score 3 = severe), in case of marked increase in hepatic echogenicity, with poor or absent visualization of the wall of the portal vein, the diaphragm and the posterior part of the right hepatic lobe
Time frame: Retrospective (years 2000-2023)
Historical Nonalcoholic Fatty Liver Disease Prevalence Change in Pediatric Celiac Disease Patients
A comparison between pre and post gluten free diet (1 year of diet) Nonalcoholic Fatty Liver Disease evidence will be performed in all historical pediatric celiac disease patients who have undergone an abdominal ultrasound both before and after the introduction of the GFD. Hepatic steatosis will be ascertained by ultrasound examination and classified as follows: absent (score 0), present (score 1). Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical Nonalcoholic Fatty Liver Disease Degree Change in Pediatric Celiac Disease Patients
A comparison between pre and post gluten free diet (1 year of diet) Nonalcoholic Fatty Liver Disease degree will be performed in all historical pediatric celiac disease patients who have undergone an abdominal ultrasound both before and after the introduction of the GFD. Hepatic steatosis will be ascertained by ultrasound examination and classified as reported in outcomes 3 and 4. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical Aspartate Aminotransferase changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Aspartate Aminotransferase will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical Alanine Aminotransferase changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Alanine Aminotransferase will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical Albuminemia changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Albuminemia will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical total cholesterolemia changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Total cholesterolemia will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical fasting glycemia changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Fasting glycemia will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
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Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical insulinemia changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Insulinemia will be assessed in all subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
HOMA-IR Index will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical anti-gliadin immunoglobulin A changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Anti-gliadin immunoglobulin A will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical anti-gliadin immunoglobulin G changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Anti-gliadin immunoglobulin G will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical anti-tissue transglutaminase immunoglobulin A changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Anti-tissue transglutaminase immunoglobulin A will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical anti-tissue transglutaminase immunoglobulin G changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Anti-tissue transglutaminase immunoglobulin G will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical anti-endomysial antibodies immunoglobulin A changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Anti-endomysial antibodies immunoglobulin A will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Historical human leukocyte antigen status in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Human leukocyte antigen status will be assessed in all subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023)
Historical Marsh duodenal histological classification changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Marsh duodenal histological classification will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet, and who underwent gastroscopy both before and after start of gluten-free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Duodenal eosinophils infiltration changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients
Duodenal eosinophils infiltration will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet, and who underwent gastroscopy both before and after start of gluten-free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Body Mass Index changes between pre and post gluten free diet in Nonalcoholic Fatty Liver Disease Pediatric Celiac Disease Patients.
Body mass index will be assessed in all historical subjects who had or did not have a change in liver steatosis degree between pre and post 1 year of gluten free diet. Difference will be considered significant for p\<0.05.
Time frame: Retrospective (years 2000-2023); 1 year of diet
Intestinal permeability markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: zonulin
Zonulin serum levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The assay will be carried out using the enzyme-linked immunosorbent assay method, with commercially available kits, following the manufacturers' instructions.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Intestinal permeability markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: occludin
Occludin serum levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The assay will be carried out using the enzyme-linked immunosorbent assay method, with commercially available kits, following the manufacturers' instructions.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Intestinal permeability markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: anti-occludin antibodies
Anti-occludin antibodies serum levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The assay will be carried out using the enzyme-linked immunosorbent assay method, with commercially available kits, following the manufacturers' instructions.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Intestinal permeability markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: claudin 1
Claudin 1 serum levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The assay will be carried out using the enzyme-linked immunosorbent assay method, with commercially available kits, following the manufacturers' instructions.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Intestinal permeability markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: anti-claudin 1 antibodies
Anti-claudin 1 antibodies serum levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The assay will be carried out using the enzyme-linked immunosorbent assay method, with commercially available kits, following the manufacturers' instructions.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Duodenal mucosa damage markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: Intestinal Fatty Acid Binding Protein 2
Intestinal Fatty Acid Binding Protein 2 serum levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The assay will be carried out using the enzyme-linked immunosorbent assay method, with commercially available kits, following the manufacturers' instructions.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Duodenal mucosa damage markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: lipopolysaccharide
Lipopolysaccharide serum levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The assay will be carried out using the enzyme-linked immunosorbent assay method, with commercially available kits, following the manufacturers' instructions.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Duodenal mucosa damage markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: lipopolysaccharide binding protein
Lipopolysaccharide binding protein serum levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The assay will be carried out using the enzyme-linked immunosorbent assay method, with commercially available kits, following the manufacturers' instructions.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Inflammatory markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: tumor necrosis factor a
Tumor necrosis factor a levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The measurement of inflammation markers will be performed by intracellular staining with flow cytometric method on peripheral blood mononuclear cells (PBMC) extracted from patients' blood samples.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Inflammatory markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: interleukin 1b
Interleukin 1b levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The measurement of inflammation markers will be performed by intracellular staining with flow cytometric method on peripheral blood mononuclear cells (PBMC) extracted from patients' blood samples.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Inflammatory markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: interleukin 6
Interleukin 6 levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The measurement of inflammation markers will be performed by intracellular staining with flow cytometric method on peripheral blood mononuclear cells (PBMC) extracted from patients' blood samples.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Inflammatory markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: interleukin 12
Interleukin 12 levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The measurement of inflammation markers will be performed by intracellular staining with flow cytometric method on peripheral blood mononuclear cells (PBMC) extracted from patients' blood samples.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]
Inflammatory markers changes in newly diagnosed Pediatric Celiac Disease Patients before and after gluten free diet: interleukin 18
Interleukin 18 levels changes will be assessed before and after 6 months of gluten free diet in newly diagnosed pediatric celiac disease patients both with and without liver steatosis. Difference will be considered significant for p\<0.05. The measurement of inflammation markers will be performed by intracellular staining with flow cytometric method on peripheral blood mononuclear cells (PBMC) extracted from patients' blood samples.
Time frame: 6 months [from T0 (before start of gluten-free diet) to T1 (after 6 months of gluten-free diet)]