The goal of this observational study is to learn about functional heterogeneity of immune cells before and after cardiopulmonary bypass (CPB) in children with congenital heart disease (CHD). The main question\[s\] it aims to answer are: * Does CPB cause immune paralysis after CHD surgery? * How does the functional heterogeneity of immune cells change before and after CPB in children undergoing CHD surgery? Participants will receive 3ml of peripheral blood before CPB, 2 hours after CPB, and 3 days after CPB, and the peripheral blood samples will be sequenced by single cell to explore the immune heterogeneity before and after CPB.
CPB-induced immune paralysis is the main cause of postoperative infection in CHD, which seriously affects the prognosis of children. The underlying mechanism of CPB-induced immune paralysis has not been fully determined. This study intends to use scRNA-seq to describe gene transcription profiles of immune cells in peripheral blood of children undergoing CHD surgery before and after CPB, and explore the differences within and between different immune cell populations including T cells and the changes of immune cells in different sequences. To fill the gap in the study of immune mechanism in CPB-induced immune paralysis microenvironment at single-cell resolution.
Study Type
OBSERVATIONAL
Enrollment
3
Children's Hospital, Zhejiang University School of Medicine
Hangzhou, Zhejiang, China
Expression of genes
The gene expression in single cells of peripheral blood samples was detected
Time frame: From enrollment to the end of CPB at 3 days
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.