BCAAs, Muscle Protein Synthesis and Glucose Uptake

Wageningen University24 enrolled

Overview

The present study aims to quantify the insulin sensitivity and the skeletal muscle protein synthetic response to consuming a test drink containing all amino acids or all amino acids except for BCAAs.

Twenty-eight healthy, young volunteers will receive a stable isotope tracer infusion (7.5h) combined with repeated blood and muscle sampling, to measure muscle protein synthesis rate in the postabsorptive state following ingestion of a drink containing all amino acids (n=14) or all amino acids except for BCAAs (n=14).

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

DOUBLE

Enrollment

Conditions

Healthy

Interventions

Ingestion of an amino acid supplementDIETARY_SUPPLEMENT

Oral intake of a supplement containing amino acids

Eligibility

Sex: ALLMin age: 18 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Aged from 18-35 years * 18.5 \< Body mass index \< 30 kg·m2 * Recreationally active (performing non-competitive physical exercise at least one time per week for minimally 30 minutes) Exclusion Criteria: * Smoking * Diabetes (Type 1, Type 2, or genetic form of diabetes) * Any diagnosed cardiovascular (heart) disease or high blood pressure (≥140 mmHg systolic and/or ≥90 mmHg diastolic) * Chronic use of any prescribed or over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices) * Known allergy to lidocaine * Regular use of dietary protein and/or amino acid supplements (\>3 times per week) * Currently involved in a structured progressive resistance training programme (\>3 times per week) * A personal or family history of thrombosis (clots), epilepsy, seizures, or schizophrenia. * Any previous motor disorders or disorders in muscle and/or lipid metabolism * Presence of an ulcer in the stomach or gut and/or strong history of indigestion * Known severe kidney problems * Pregnant or breastfeeding * Unable to give consent

Locations (1)

Wageningen University and Research

Wageningen, Gelderland, Netherlands

Outcomes

Primary Outcomes

Postprandial muscle protein synthesis

Muscle protein synthesis rate (FSR) prior to and following ingestion of an amino acid drink

Time frame: 4 hours

Secondary Outcomes

Forearm muscle glucose uptake

Insulin sensitivity, measured as forearm glucose uptake, prior to and following ingestion of an amino acid drink

Time frame: 7.5 hours

Whole-body insulin sensitivity

Whole-body insulin sensitivity, calculated using a modified Matsuda index

Time frame: Four hours following ingestion of an amino acid drink

Forearm amino acid kinetics

Plasma phenylalanine kinetics using a stable isotope tracer infusion

Time frame: Prior to and for 4 hours following amino acid intake

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.