Acute Physiological Effects of Greek Traditional Dancing

University of Thessaly20 enrolled

Overview

In Greece, people of different age groups, including young children to older adults, are involved in traditional dance. To date, the well-know benefits of dancing include entertainment, socialization and increased physical activity. However, the acute effects of Greek traditional dancing on health, physical performance and muscle damage indices remain largely unknown. Therefore, the aim of this project is to evaluate the acute effect of Greek traditional dancing on health-, physical performance-, and muscle damage-related parameters by considering the impact of dancing tempo (slow vs moderate vs fast). In a crossover repeated measures design 10 pre- and 10 post-menopausal women will participate in the three dancing sessions of different tempo in a random order.

This study aims at determining the acute effect of Greek traditional dancing on health- and physical performance-related parameters by considering the impact of dancing tempo. Ten pre- and ten postmenopausal women meeting the inclusion criteria will be assigned to a crossover trial. Initially, participants will undergo baseline testing including (i) anthropometrics, (ii) body composition, (iii) physical performance, (iv) physical activity level and (v) dietary intake. After baseline measurements, a 7-day familiarization period will take place, during which participants will practice daily on the three experimental dances. Each dance will be characterized by a different tempo (beats per minute) so that there will be one dance from each tempo category (i.e. slow, moderate and fast). After familiarization, participants will execute in a random order the three dancing sessions, on separate days. Each dancing session will consist of one dance (of either slow or moderate or fast tempo) lasting \~ 3- 4 minutes. During each dance heart rate, oxygen consumption and activity intensity will be continuously monitored. Before and immediately after the dance systolic and diastolic blood pressure, perceived exertion and blood lactate will be measure. In addition, before each dance and at 24 and 48 hours post-dance muscle soreness, muscle strength and resting metabolic rate will be measured and a resting blood sample will be drawn for the assessment of inflammatory and oxidative stress markers.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

Eligibility

Sex: FEMALEMin age: 20 YearsMax age: 80 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Premenopausal and postmenopausal women. * Abstain from vigorous physical activity and/or exercise during the last (at least) 6 months prior to the study. * Free of musculoskeletal diseases and injuries. * Free of cardiometabolic diseases. * Free of mental health disorders * No body weight loss \>10% over the last 6 months prior to the study * Non-smokers Exclusion Criteria: * Perimenopause and menopause women * Participation vigorous physical activity and/or exercise during the last (at least) 6 months prior to the study * Presence of musculoskeletal diseases and injuries. * Presence of cardiometabolic diseases. * Presence of mental health disorders * Body weight loss \>10% over the last 6 months prior to the study * Smokers

Outcomes

Primary Outcomes

Change in blood pressure

Systolic and diastolic blood pressure will be measured using a blood pressure cuff

Time frame: At baseline and immediately after the dance

Change in perceived exertion

Perceived exertion will be rated using the Borg Rating of Perceived Exertion scale, a numerical scale that ranges from 6 (no exertion) to 20 (maximum effort).

Time frame: At baseline and immediately after the dance

Change in blood lactate

Blood lactate will be measured on a portable, automatic lactate analyser using the relevant strips

Time frame: At baseline and immediately after the dance

Change in resting metabolic rate

Resting metabolic rate (kcal) will be assessed using a portable open-circuit indirect calorimeter with a ventilated hood system

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in delayed-onset of muscle soreness (DOMS)

DOMS of the knee extensors and flexors of both limbs will be evaluated by palpation of the relaxed muscle's belly and distal region with participants rating the perceived soreness on a visual analogue scale ranging from 1 to 10.

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in maximal isometric voluntary contraction

Maximal isometric voluntary contraction of the knee extensors and flexors of both limbs will be assessed using an isokinetic dynamometer.

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in joint range of motion

Knee joint range of motion will be assessed using a manual goniometer

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in functional performance

Functional performance will be assessed using the movement-based screening tool functional movement screen (FMS)

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in white blood cell count

White blood cell count will be measured using an automatic blood analyzer

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in granulocyte count

Granulocyte count will be measured using an automatic blood analyzer

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in lymphocytes

Lymphocytes will be measured using an automatic blood analyzer

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in monocytes

Monocytes will be measured using an automatic blood analyzer

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in creatine kinase concentration

Creatine kinase will be measured using an automatic blood analyzer and commercially available kits

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in reduced glutathione

Reduced glutathione will be measured spectrophotometrically in red blood cells

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in oxidized glutathione

Oxidized glutathione will be measured spectrophotometrically in red blood cells

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in catalase activity

Catalase activity will be measured spectrophotometrically in red blood cells

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in protein carbonyls

Protein carbonyls will be measured spectrophotometrically in red blood cells

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in total antioxidant capacity

Total antioxidant capacity will be measured spectrophotometrically in plasma

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in heart rate

Heart rate will be continuously monitored using heart rate sensors

Time frame: Through dance session completion, an average of 30 minutes

Change in oxygen consumption

Oxygen consumption will be continuously monitored using a portable metabolic system.

Time frame: Through dance session completion, an average of 30 minutes

Change in physical activity

The number of steps performed will be assessed using an accelerometer (ActiGraph Gt3x)

Time frame: Through dance session completion, an average of 30 minutes

Secondary Outcomes

Change in red blood cell count

Red blood cell count will be measured using an automatic blood analyzer

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in hematocrit

Hematocrit will be measured using an automatic blood analyzer

Time frame: At baseline and at 24 hours and 48 hours after the dance

Change in hemoglobin

Hemoglobin will be measured using an automatic blood analyzer

Time frame: At baseline and at 24 hours and 48 hours after the dance

Acute Physiological Effects of Greek Traditional Dancing

Overview

Conditions

Interventions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes