Recovery Kinetics Following a Soccer Training in Middle-aged Males

N/ACompletedNCT06260215

University of Thessaly11 enrolled

Overview

This study aims at investigating the recovery kinetics of skeletal muscle damage, neuromuscular fatigue and performance following a single soccer trainning session in middle aged males. The participants will perform a soccer training session \[A single training session including 60 minutes of warm up, soccer technical exercises and small-sided game\] and a cotrol trial (No intervention included, only daily measurements) in randomized, repeated measures, crossover design. Assesesments related to skeletal muscle damage, performance and neuromuscular fatigue will be executed before the training session and daily for four consecutive days after training.

Eleven male middle-aged participants will be included in this study. Participants will be initially informed about the main goals of study as well as the associated risks and benefits and then will provide their signed consent form. At baseline, they will undergo assessment of their anthropometrics (body mass, height, BMI, WHR), body compotition (using DXA), physical contition level (peak oxygen consumption, VO2peak), haemodynamic profile (diastolic and diastolic blood pressure) and daily dietary intake.Thereafter, they will participate in two experimental trials (Control trial and Soccer trial) in a randomized, crossover, repeated measure design: 1) ST (Soccer Trial): Participants in this trial will perform a soccer training session (60 min) consisted of warm-up, technical exercises and small-sided game, CT (Control Trial): Participants in this trial will only execute the evaluations daily in the dependent variables (they will not receive any intervention). Before each trial, the participants will provide a resting blood sample (for the determination of white blood cells, granulocyte cells, monocyte cells, lymphocyte cells, Hemeatocrit, Hemoglobin, red blood cells and platelets, total antioxidant capacity, glutathione and creatine kinase activity) and undergo assessments of their delay onset muscle soreness (DOMS), maximal voluntary isometric contraction (MVIC) of knee extensors and flexors (by isokinetic dynamometer), height and power of countermovement jump (CMJ-using force platform), 10m and 30m sprint time (will be evaluated using light cells). A 7-day wash-out period will apllied between ST and CT. The blood sample will be collected before and 24, 48 and 72 hours post-taining. Also, the evaluation of DOMS, MVIC, sprinting performance, power and jump height (by CMJ) will be evaluated before and 24, 48, 72 and 96 hours post training.

Study Type

INTERVENTIONAL

Outcomes

Primary Outcomes

Change in White blood cell count

White blood cell count will be measured using an automatic blood analyzer

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in Granulocyte cell count

Change in White blood cell count will be measured using an automatic blood analyzer

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in Platelets cell count

Platelets cell count will be measured using an automatic blood analyzer

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in Monocyte cell count

Monocytes cell count will be measured using an automatic blood analyzer

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in Lymphocyte cell count

Lymphocyte cell count will be measured using an automatic blood analyzer

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in percentage of Hematocrit level

The percentage of Hematocrit level will be measured using an automatic blood analyzer

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change Red blood cells count

Red blood cells count will be measured using an automatic blood analyzer

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in Hemoglobin level

Hemoglobin level will be measured using an automatic blood analyzer

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in Creatine kinase activity

Creatine kinase activity will be measured in plasma

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in Total antioxidant capacity

Total antioxidant capacity will be measured in plasma

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in Glutathione concentration in blood

Glutathione concentration will be measured in red blood cells

Time frame: At baseline, at 24, 48 and 72 hours after soccer training

Change in countermovement jump height

countermovement jump height will be measured using a force platform

Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

Change in Peak power during coutermovement jump test

The peak power will be measured using a force platform

Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

Change in isometric peak torque of knee extensors and flexors

Isometric peak torque will be assessed on an isokinetic dynamometer

Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

Change in sprint time of 10 m

10m sprint time will be assessed using light cells

Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

Change in sprint time of 30 m

30m sprint time will be assessed using light cells

Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

Change in delayed onset of muscle soreness

Muscle soreness will be assessed by palpation of muscle belly and the distal region

Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

Secondary Outcomes

Dietary intake

Dietary intake will be assesed over a 7-day period using diet recalls

Time frame: At baseline

Peak Maximal oxygen consumption (Peak VO2)

Peak VO2 will be estimated by open circuit spirometry via breath by breath method

Time frame: At baseline

Body Mass

Body Mass will be measured on a beam balance with stadiometer

Time frame: At baseline

Body Height

Body Height will be measured on a beam balance with stadiometer

Time frame: At baseline

Body Fat

Body Fat will be measured by using Dual-emission X-ray absorptiometry

Time frame: At baseline

Lean body mass

Lean body mass will be measured by using Dual-emission X-ray absorptiometry

Time frame: At baseline

Bone mass density

Bone mass density will be measured by using Dual-emission X-ray absorptiometry

Time frame: At baseline

Bone mass content

Bone mass content will be measured by using Dual-emission X-ray absorptiometry

Time frame: At baseline

Field activity during the soccer training

The field activity will be continuously monitored during the soccer training using global positioning system (GPS)

Time frame: During the 60 minutes soccer training (ST) session (72 hours after baseline measurements)

NCT06260215 - Recovery Kinetics Following a Soccer Training in Middle-aged Males | Crick | Crick