Effect of Nonsurgical Periodontal Therapy on Salivary Biomarkers Related to Bone Turnover in Smoker and Nonsmoker Periodontitis Patients

Inclusion Criteria: * participants will be systemically healthy without history of any systemic diseases. * hadn't underwent periodontal treatment during the previous 6 months and not taken medication during the previous 3 months. * present with at least 20 teeth, those who only diagnosed with 4-6 mm probing pocket depth, generalized ,unstable periodontitis who had bone loss \> 30% , probing probing pocket depth at ≥4mm . Periodontitis cases were defined if clinical attachment loss was detected at ≥2 non- adjacent teeth or when buccal (facial) showed clinical attachment loss ≥3mm associated with probing pocket depth \>3mm at ≥ 2 teeth . * The patients in the smokers group will be current smokers ( cigarette smokers) with smoking habit for over 3 years Exclusion Criteria: * Subjects have any intraoral plaque retentive factors such as those wearing partial denture, crown and bridges, and orthodontic appliances ,furcation involved teeth, overhang filling, tooth anomaly. * receiving antibiotic therapy during the previous 3 months. * those with systemic diseases such as diabetes mellitus, cardiovascular or renal diseases, etc…., pregnant or lactating women, not willing to participate or continue to conduct the study.