The goal of this project is to learn about differences in bone development between children with and without type-1 diabetes (T1D). The main questions this study aims to answer are: 1. Assess how and when sex-specific bone developmental trajectories in the leg and arm will differ between children with T1D and control cohorts relative to the critical period of rapid skeletal growth in puberty. It is hypothesized that children with T1D will have inferior bone development, particularly lower gains in bone strength. 2. Assess why bone trajectories differ between T1D and control cohorts by identifying the role of body composition, site-specific muscle force and physical activity on differences in bone properties in female and male children with and without T1D. It is hypothesized that children with T1D will have lower gains in lean mass, muscle force, number of daily bone impacts and minutes of moderate-vigorous physical activity and will be associated with inferior gains in bone development. 3. Assess why T1D may impair sex-specific bone development by exploring the role of disease-related factors (e.g., duration, glucose control, hormones and markers of bone turnover) and fracture history on bone trajectories of children with T1D. It is hypothesized that longer exposure to T1D, poorer glucose control, alterations in hormones, lower bone formation markers and higher history of fracture will be negatively associated with bone trajectories of children with T1D. Participant's physical growth, bone growth, muscle strength, physical activity and nutrition habits will be assessed and followed up annually for up to 4 years.
The Canadian Bone Strength Development Study is a multi-site project examining differences in bone development between children with and without type-1 diabetes. Research for this study will be conducted at the University of Saskatchewan, University of Calgary, The Hospital for Sick Children (SickKids) and The Children's Hospital of Eastern Ontario (CHEO). 204 children (50% female) will be included. Participants will make 4 annual visits to the laboratory. During each laboratory visit, anthropometric measurements (e.g., height and weight), bone strength and microarchitecture, muscle strength, nutrition and physical activity will be assessed. Investigators will compare between group differences in bone growth trajectories using multilevel models.
Study Type
OBSERVATIONAL
Enrollment
204
University of Saskatchewan
Saskatoon, Saskatchewan, Canada
RECRUITINGTrabecular Thickness (μm)
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Bone Strength (Failure load)
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Total, Cortical and Trabecular Bone Area (mm^2)
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Total, Cortical and Trabecular Bone Density (mg HA/cm^3)
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Cortical Thickness (μm)
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Cortical Porosity
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Trabecular Bone Volume Fraction (%)
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Trabecular Bone Number (1/mm)
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Trabecular Bone Separation (μm)
Assessed by high-resolution peripheral quantitative computed tomography (HRpQCT).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Total Body and Hip Bone Mineral Content (g)
Assessed by Dual-energy X-ray absorptiometry (DXA).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Total Body and Hip Areal Bone Mineral Density (g/cm^2)
Assessed by Dual-energy X-ray absorptiometry (DXA).
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Age from Peak Height Velocity (years)
Body mass, height and sitting height will be used to estimate age from peak height velocity.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Pubertal Development
Pubertal development will be determined using Tanner Stages (self-assessed). Participants will be asked to look at drawings of pubic hair and genital or breast development and rate what drawing best reflects their current stage of development. Each set of drawings is associated with a pubertal stage (1-5). Stage 1 indicates pre-pubertal status, stage 2-3 indicates early pubertal status, stage 4 indicates pubertal status and stage 5 indicates post-pubertal status.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Long Jump (m)
The furthest distance an individual can jump while starting from a standing position.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Hand Grip (N)
The highest amount of force one can develop by squeezing their hand. Force is measured using a hand grip dynamometer.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Bone Impacts
Estimate of bone impact activities using a waist-worn accelerometer monitored over 7 days.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Physical Activity (minutes/day)
Estimate of daily moderate-to-vigorous physical activity using a waist-worn accelerometer monitored over 7 days.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Food Frequency Questionnaire
Estimate of daily intakes of calcium, protein and vitamin D. Estimates will be derived using the Food Frequency Questionnaire. Participants will be asked to recall their consumption of various foods over the last 6 months and rate their intake on a scale from Never to consuming the food item 5-6 per week. These ratings are then used to estimate the daily intake of calcum, protein and vitamin D.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Health
For children with type-I diabetes, investigators will record the onset of T1D (years), insulin regimen and various measures of glycemic control (e.g., HbA1c) using open-ended questionnaires and participants' medical records.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Osteocalcin
Biochemical assessment of bone formation.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Procollagen type I N-propeptide (P1NP-N)
Biochemical assessment of bone formation.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
C-terminal telopepide (CTx)
Biochemical assessment of bone resorption.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Sclerostin
Biochemical assessment of bone resorption.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Insulin growth factor-1
Biochemical assessment of hormones.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
Alkaline phosphatase
Biochemical assessment of hormones.
Time frame: Baseline, year 1 follow-up, year 2 follow-up, year 3 follow-up
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