GCF Levels of Chemokines in Periodontitis

Inclusion Criteria: * Have at least 20 natural teeth, excluding third molars. * Periodontitis patients had at least two non-adjacent sites per quadrant with probing depth (PD) ≥ 5 mm and clinical attachment level (CAL) ≥ 5 mm with gingival inflammation, and alveolar bone loss affecting \>30% of the teeth, as detected on clinical and radiographical examinations. * Periodontally healthy control group had no sign of gingival inflammation, no PD \> 3mm and no evidence of attachment or bone loss Exclusion Criteria: * History of systemic disease. * Regular use of any drugs which can effect the immune system or inflammatory response in the 6 months preceding the start of the study. * Periodontal treatment during last 6 months that could affect periodontal status. * Smoking. * History of radiotherapy or chemotherapy. * Current pregnancy, lactation or menopause.