The aim of this study was to investigate the effects of the consumption of cookies produced by adding the unripe part of carob fruit, which has high insoluble fiber, polyphenol and antioxidant content, on the oxidative parameters (TAS and MDA), intestinal permeability (zonulin, GIP) and microbiota of celiac patients and to develop new products containing unripe carob fruit for celiac patients in this direction.
Between 01.02.2022 and 30.05.2022 by Specialist Dr. Orhan SEZGİN The study will be conducted with 30 volunteers who present to the Mersin University Faculty of Medicine Hospital Gastroenterology Department in Mersin.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
SUPPORTIVE_CARE
Masking
DOUBLE
Enrollment
30
The standard cookies and test cookies (with carob addition) to be used in the study will be produced by Dr. Lecturer Özlem ÖZPAK AKKUŞ at Toros University Nutrition Principles Laboratory. Prof. Dr. Özlem ÖZPAK AKKUŞ will produce the cookies. For standard cookies, 1 egg, 325 g gluten-free flour (Söke flour), 75 grams butter, 50 grams sugar, 10 grams baking powder, 10 grams vanilla will be added and the prepared bread mixture will be divided into 50 equal parts. To make cookies with the addition of unripe carob, the same recipe as the standard cookie will be used to make the test cookie with the addition of unripe carob fruit flour, divided into 50 equal parts, and the amount of unripe carob fruit flour will be added by calculating the daily fiber consumption amount of the patients participating in the study to be 25 g.
Examination of between group differences regarding serum Total Antioxidant Capacity (TAC)
Examination of intergroup differences of the concentration of the serum total antioxidant capacity for the determination of antioxidant capacity status in celiac diseases
Time frame: First day at the beginning of the study
Examination of between group differences regarding serum Malondialdehyde (MDA)
Examination of intergroup differences of the concentration of the serum malondialdehyde for the determination of oxidant capacity status in celiac diseases
Time frame: First day at the beginning of the study
Examination of between group differences regarding serum zonulin
Examination of intergroup differences of the concentration of the serum zonulin for the determination of intestinal permeability in celiac diseases
Time frame: First day at the beginning of the study
Comparison of between group differences regarding serum Total Antioxidant Capacity (TAC)
Comparison of between group differences of the concentration of the serum total antioxidant for the determination of antioxidant capacity status in celiac diseases
Time frame: Eight weeks after the start of the study
Comparison of between group differences regarding serum Malondialdehyde (MDA)
Comparison of between group differences of the concentration of the serum malondialdehyde for the determination of oxidant capacity status in celiac diseases
Time frame: Eight weeks after the start of the study
Comparison of between group differences regarding serum zonulin
Comparison of between group differences of the concentration of the serum zonulin for the determination of intestinal permeability in celiac diseases
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Time frame: Eight weeks after the start of the study