The goal of this observational study is to learn about intestinal microbiome structure and function in individuals who have undergone a pancreatoduodenectomy and compare to healthy matched controls. The primary objectives of the study are: 1. To explore and describe any differences in the gut microbiota especially Shannon diversity index 2. To conduct functional profiling by exploring and describing any differences in functional metabolites produced in the gut in people having had pancreatoduodenectomy greater than 6 months ago compared to healthy matched controls. Participants will be asked to complete the following: * Three-day food, bowel and medication diary (see Protocol appendix 5) * Gastrointestinal Symptom Rating Scale (see Protocol appendix 6) * Quality of life questionnaire (see Protocol appendix 7) * Stool sample test using Microba Insight TradeMark (a small swab is taken from soiled toilet paper, sealed in a room-temperature storage capsule and mailed to the testing laboratory)
Study Type
OBSERVATIONAL
Enrollment
20
Department of Nutrition and Dietetics, Royal Prince Alfred Hospital
Camperdown, New South Wales, Australia
RECRUITINGComparison of microbiota profile between post-surgical participants and healthy controls.
Stool samples will be analysed to compare the Shannon diversity index (calculated by taking the number of each microbial species, the proportion each species is of the total number of individuals, summed by the proportion times the natural log of the proportion for each species) between participant groups.
Time frame: During data collection, 3 days
Compliance with 3-day food dairies
Compliance with 3-day food dairies (completion rate of food diaries)
Time frame: From enrollment to the collection of data from participants, 3 days
Pre-paid return rates
Pre-paid return rates (proportion (number) of envelopes returned)
Time frame: From enrollment to the collection of data from participants, 3 days
Ease of matching
Ease of matching (ability to match surgical patients to controls based on age, gender, body mass index, smoking status)
Time frame: Enrollment period, up to 12 months
Shannon diversity index
Shannon diversity index (calculated by taking the number of each microbial species, the proportion each species is of the total number of individuals, summed by the proportion times the natural log of the proportion for each species)
Time frame: During data collection period, 3 days
Gastrointestinal symptoms
Gastrointestinal symptoms (total number of bowel motions)
Time frame: During data collection period, 3 days
Protein:fibre ratio of diet
Protein:fibre ratio of diet (average grams of protein consumed divided by grams of fibre consumed)
Time frame: During data collection period, 3 days
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