In this study, the investigators will include PCOS patients who meet the trial criteria, introduce participants to the content of this study, and invite participants to participate. The immune function of peripheral blood samples of PCOS patients was detected by flow cytometry. Participants were further treated with metformin for 6 months and followed up after the intervention. The objective of this study was to investigate immune markers related to the efficacy of metformin in PCOS patients and to predict the efficacy of metformin in PCOS patients using immune function.
In this study, peripheral blood samples of PCOS patients were collected before and after intervention. Flow cytometry was used to detect the antigenic antibody reaction between the surface differentiation antigen of lymphocyte membrane and fluorescently labeled antibodies in human peripheral blood, and the unlabeled cells were dissolved. The number and percentage of cell groups expressing different markers were counted by the fluorescence detection system on flow cytometry.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
TREATMENT
Masking
NONE
Enrollment
100
Take metformin for six months.
Department of Endocrinology, Shanghai Tenth People's Hospital
Shanghai, China
RECRUITINGThe number of cell groups expressing different markers were counted by the fluorescence detection system on flow cytometry.
Flow cytometry was used to detect the antigenic antibody reaction between the surface differentiation antigen of lymphocyte membrane and the fluorescently labeled antibody in human peripheral blood, and the unlabeled cells were dissolved.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
The percentage of cell groups expressing different markers were counted by the fluorescence detection system on flow cytometry.
Flow cytometry was used to detect the antigenic antibody reaction between the surface differentiation antigen of lymphocyte membrane and the fluorescently labeled antibody in human peripheral blood, and the unlabeled cells were dissolved.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Homeostasis model assessment of insulin resistance
insulin resistance index
Time frame: Within 7 days of enrollment.
body mass index
body mass index (kg/m2)
Time frame: Within 7 days of enrollment.
menstrual frequency
number of menstruation in a year
Time frame: Within 7 days of enrollment.
fasting glucose
fasting glucose (mmol/L)
Time frame: Within 7 days of enrollment.
fasting insulin
fasting insulin (mmol/L)
Time frame: Within 7 days of enrollment.
Total cholesterol
Total cholesterol (mmol/L)
Time frame: Within 7 days of enrollment.
Triglycerides
Triglycerides (mmol/L)
Time frame: Within 7 days of enrollment.
HDL-c
HDL-c (mmol/L)
Time frame: Within 7 days of enrollment.
LDL-c
LDL-c (mmol/L)
Time frame: Within 7 days of enrollment.
total testosterone
total testosterone (nmol/L)
Time frame: Within 7 days of enrollment.
free testosterone
free testosterone (nmol/L)
Time frame: Within 7 days of enrollment.
Sex hormone-binding globulin
Sex hormone-binding globulin (nmol/L)
Time frame: Within 7 days of enrollment.
Androstenedione
Androstenedione (ng/ml)
Time frame: Within 7 days of enrollment.
Dehydroepiandrosterone
Dehydroepiandrosterone (ug/dl)
Time frame: Within 7 days of enrollment.
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