In this study, the investigators will include PCOS patients and healthy women who meet the trial criteria from the clinical research centers of 3 hospitals, introduce the content of this study to participants, and invite participants to participate. Flow cytometry was used to detect the immune function of peripheral blood samples of PCOS patients, to clarify the immune function characteristics of PCOS patients, and to try to establish an evaluation method of the immune function of PCOS patients.
PCOS patients and healthy women were recruited in outpatient clinics of three hospitals, and peripheral blood was retained for flow cytometry after completing relevant examinations. In this study, flow cytometry was used to detect the antigenic antibody reaction between the surface differentiation antigen of lymphocyte membrane and fluorescence-labeled antibody in human peripheral blood, and the unlabeled cells were dissolved. The number and percentage of cell groups expressing different markers were counted by the fluorescence detection system on flow cytometry.
Study Type
OBSERVATIONAL
Enrollment
162
Flow cytometry was used to detect the immune function of peripheral blood samples of PCOS patients.
Department of Endocrinology, Shanghai Tenth People's Hospital
Shanghai, China
The number of cell groups expressing different markers were counted by the fluorescence detection system on flow cytometry.
Flow cytometry was used to detect the antigenic antibody reaction between the surface differentiation antigen of lymphocyte membrane and the fluorescently labeled antibody in human peripheral blood, and the unlabeled cells were dissolved.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
The percentage of cell groups expressing different markers were counted by the fluorescence detection system on flow cytometry.
Flow cytometry was used to detect the antigenic antibody reaction between the surface differentiation antigen of lymphocyte membrane and the fluorescently labeled antibody in human peripheral blood, and the unlabeled cells were dissolved.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Homeostasis model assessment of insulin resistance
insulin resistance index
Time frame: Within 7 days of enrollment.
body mass index
body mass index (kg/m2)
Time frame: Within 7 days of enrollment.
menstrual frequency
number of menstruation in a year
Time frame: Within 7 days of enrollment.
fasting glucose
fasting glucose (mmol/L)
Time frame: Within 7 days of enrollment.
fasting insulin
fasting insulin (mmol/L)
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Time frame: Within 7 days of enrollment.
Total cholesterol
Total cholesterol (mmol/L)
Time frame: Within 7 days of enrollment.
Triglycerides
Triglycerides (mmol/L)
Time frame: Within 7 days of enrollment.
HDL-c
HDL-c (mmol/L)
Time frame: Within 7 days of enrollment.
LDL-c
LDL-c (mmol/L)
Time frame: Within 7 days of enrollment.
total testosterone
total testosterone (nmol/L)
Time frame: Within 7 days of enrollment.
free testosterone
free testosterone (nmol/L)
Time frame: Within 7 days of enrollment.
Sex hormone-binding globulin
Sex hormone-binding globulin (nmol/L)
Time frame: Within 7 days of enrollment.
Androstenedione
Androstenedione (ng/ml)
Time frame: Within 7 days of enrollment.
Dehydroepiandrosterone
Dehydroepiandrosterone (ug/dl)
Time frame: Within 7 days of enrollment.