Acute Physiological Responses to Twice Daily Blood Flow Restriction Training

Inclusion Criteria: * Age 18 - 55 years * Must not have performed regular lower-limb resistance training for the last 6 months (participants must have performed \< 1 lower-limb resistance training session per week over the previous 6 months) * DMRC Staff member (civilian or service personnel) Exclusion Criteria: * Any medical contraindication related to BFR\*. * Any current lower-limb musculoskeletal injury * Restricted range of movement. i.e. Chronically locked knee or fixed flexion deformity. * Any physical impairment or co-morbidities (including cardio-vascular disease) precluding the safe participation in the intervention and/or assessment procedures * Non-musculoskeletal or serious pathological condition (i.e. Inflammatory arthropathy, infection or tumour). * Use of any medication which may contraindicate the performance of BFR or influence blood biomarkers of EIMD or inflammation. * Use of any performance enhancing supplements or ergogenic aids such as creatine monohydrate. * Any individual who is known to be currently pregnant \*Medical-based Exclusion Criteria (based on clinical contraindications to performing BFR): * History of cardiovascular disease (hypertension, peripheral vascular disease, thrombosis/embolism, ischaemic heart disease, myocardial infarction). * History of the following musculoskeletal disorders: rheumatoid arthritis, avascular necrosis or osteonecrosis, severe osteoarthritis. * History of the following neurological disorders: Peripheral neuropathy, Alzheimer's disease, amyotrophic lateral sclerosis, Parkinson's disease, severe traumatic brain injury. * Varicose veins in the lower-limb. * Acute viral or bacterial upper or lower respiratory infection at screening. * Known or suspected lower limb chronic exertional compartment syndrome (tourniquet raises intra-compartmental muscle pressure). * Postsurgical swelling. * Surgical insertion of metal components at the position of cuff inflation. * History of any of the following conditions or disorders not previously listed: diabetes, active cancer. * History of elevated risk of unexplained fainting or dizzy spells during physical activity/exercise that causes loss of balance * Increased risk of haemorrhagic stroke, exercise induced rhabdomyolysis.